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Jianshi Biotechnology (Beijing) Co., Ltd.

100 bp Plus DNA Ladder (TM1004)

The 100 bp Plus DNA Ladder is a mixture of individually prepared PCR products, comprising 12 DNA fragments. It is supplied with loading buffer and can be loaded directly onto the gel. Use 6 µl per lane. For easier visualization after electrophoresis, the 500 bp and 1 kb bands are the brightest; use approximately 100 ng per lane, while the other bands should be loaded at about 50 ng each.

Cat #	Specifications	Unit	Price
TM1004-50	1*300µL	support	¥177
TM1004-100	2*300µL	support	¥354
TM1004-200	3*3200µL	support	¥600

Keywords

Core Features

Dual-high-brightness design : Dual highlights at 500 bp and 1000 bp, enabling rapid anchoring
12 complete strips : Covers the full range of 100–1500 bp
High concentration : 100 ng/μl, with clearer bands
Ready-to-use : Sample buffer preloaded

Strip information

A total of 12 DNA fragments
Fragment size range: 100–1500 bp
Double high-brightness strip : 500 bp and 1000 bp, with 100 ng per lane loaded.
The remaining 10 bands : 50 ng per injection

Key Points of Use

Recommended 1%–2% agarose gel
Voltage: 4–10 V/cm
Standard sample volume: 3–6 μl
Compatible with conventional nucleic acid dyes

TM1004 Frequently Asked Questions

1. How many DNA fragments are included in TM1004? How were the highlighted bands designed?

This product contains 12 double-stranded DNA fragments ranging from 100 to 1500 bp; the 500-bp and 1000-bp (1 kb) bands are brightly fluorescent, with 100 ng loaded for each in a single lane, while the remaining bands are loaded at 50 ng. The dual‑anchor positioning facilitates rapid and accurate determination of fragment sizes.

2. What is the standard sample loading volume? How should the sample wells of different widths be adjusted?

Recommended loading volume: 3–6 μl per lane;

Loading rule: For every 1 mm of well width, load 1 μl; if the wells are wider, slightly increase the sample volume to prevent overloading, which can cause band smearing and broadening.

3. Does the product come pre-mixed with loading buffer, or does it require separate preparation?

This product comes pre‑mixed with loading buffer, so no additional preparation or addition is required. After thawing and mixing, it can be directly loaded onto the gel for electrophoresis, greatly simplifying the experimental procedure.

4. What are the recommended agarose gel concentration and electrophoresis voltage?

Gel: 1–2% agarose gel, capable of resolving a full range of fragments from 100 to 1500 bp with clear resolution;

Voltage: 4–10 V/cm (anode–cathode distance). Excessively high voltage can cause gel overheating and band distortion, while excessively low voltage results in slow separation.

5. What is the product concentration? How is the total DNA amount per sample calculation performed?

The product concentration is 100 ng/μl; based on a standard loading volume of 6 μl, the total DNA yield is approximately 600 ng, with 100 ng corresponding to each of the two prominent bands, thereby meeting the requirements for routine electrophoretic visualization and semi‑quantitative analysis.

6. How should the product be stored? What effects does repeated freeze–thaw cycling have?

Storage conditions: Store at low temperature; do not leave at room temperature for extended periods.

Note: Repeated freeze–thaw cycles can cause DNA degradation, blurred or missing bands, and uneven band intensities, directly compromising the accuracy of electrophoresis results. It is recommended to aliquot and store the product as needed upon receipt.

7. How can you quickly troubleshoot blurry bands, smearing, or missing bands after electrophoresis?

Buffer: Replace the electrophoresis buffer with a fresh one; an imbalance of ions in the old buffer can significantly reduce separation efficiency.

Gel: Use freshly prepared agarose gels to avoid improper gel concentrations and uneven gelation.

Product: Avoid nuclease contamination and DNA degradation caused by repeated freeze-thaw cycles.

Operation: Control the sample loading volume and verify that the positive and negative terminals are not reversed.

8. How long is the product warranty? How are quality issues handled?

This product is covered by a one-year quality warranty from the date of purchase, and all reagents have undergone rigorous quality testing. In the event of quality issues such as missing bands or degradation, please contact our after-sales service department with your proof of purchase for assistance.

9. What are the key differences between TM1004 and TM1002 and TM1003?

Number of bands: TM1004 has 12 lines, while TM1002 and TM1003 each have 7 lines;

Coverage: TM1004 covers 100–1500 bp, offering broader compatibility;

Highlight Design: TM1004 is dual‑highlighted (500 bp/1 Kb), TM1002 is single‑highlighted at 400 bp, and TM1003 is single‑highlighted at 700 bp.

Concentration: TM1004 has a concentration of 100 ng/μl, which is higher than the other two formulations.

10. What are the key precautions for nucleic acid staining and UV observation?

It must be used in conjunction with conventional nucleic acid dyes; the product itself does not contain any staining components.

After staining, visualize the sample using a UV gel imaging system to avoid prolonged UV exposure that could damage the DNA.

Tip: Use 500 bp/1 kb double‑bright bands as the exposure reference to prevent overexposure or underexposure from compromising band interpretation.

100 bp Plus DNA Ladder (TM1004)

TM1004 100 bp Plus DNA Molecular Weight Standard

Core Features

Strip information

Key Points of Use

TM1004 Frequently Asked Questions