100 bp Standard DNA Ladder (TM1003)

Cat #	Specifications	Unit	Price
TM1003-50	300 µL	support	¥160
TM1003-100	600 µL	support	¥320
TM1003-200	1200 µL	support	¥550

Keywords

Core Features

Wide coverage area : 100–1500 bp, suitable for longer fragments
Highlight and locate : The 700-bp band becomes twice as bright, enabling rapid anchoring.
Ready-to-use : Sample buffer preloaded
Quantitative precision : The DNA quantity in the bands is fixed, allowing for semi-quantitative reference.

Strip information

A total of 7 DNA fragments
Fragment size: 100, 300, 500, 700, 900, 1200, 1500 bp
700 bp band : 100 ng per injection (highlighted)
The remaining 6 bands : 50 ng per injection

Key Points of Use

Recommended 1%–2% agarose gel
Voltage: 4–10 V/cm
Standard sample volume: 3–6 μl
Compatible with conventional nucleic acid dyes

TM1003 Frequently Asked Questions

1. How many DNA fragments are included in TM1003? How should the fragment sizes and the highlighted bands be designed?

This product contains seven double-stranded DNA fragments, with sizes of 100 bp, 300 bp, 500 bp, 700 bp, 900 bp, 1200 bp, and 1500 bp, respectively. The 700-bp fragment is a bright band (100 ng per lane), while the remaining bands are each loaded at 50 ng per lane, facilitating rapid size determination and enhancing interpretation efficiency.

2. What is the standard sample loading volume? How should the sample wells of different widths be adjusted?

Recommended loading volume: 3–6 μl per lane;

Loading rule: For every 1 mm of well width, load 1 μl; if the wells are wider, slightly increase the sample volume to prevent overloading and subsequent band smearing.

3. Is the product pre-mixed with loading buffer? Does it require additional preparation and addition?

This product comes pre‑mixed with loading buffer, so no additional preparation or addition is required. After thawing and mixing, it can be directly loaded onto the gel for electrophoresis, simplifying the experimental workflow.

4. What are the recommended agarose gel concentration and electrophoresis voltage?

Gel: 1–2% agarose gel, optimized for clear separation of DNA fragments ranging from 100 to 1500 bp;

Voltage: 4–10 V/cm (distance between the positive and negative electrodes). Excessively high voltage can cause gel overheating and band distortion, while excessively low voltage results in prolonged electrophoresis times.

5. What is the product concentration? How is the total DNA amount per single loading calculated?

The product concentration is approximately 67 ng/μl; based on a standard loading volume of 6 μl, the total DNA yield is about 402 ng, sufficient to meet the requirements for routine electrophoresis visualization and semi‑quantitative analysis.

6. How should the product be stored? What effects does repeated freeze–thaw cycling have?

Storage conditions: Store at low temperature; do not leave at room temperature for extended periods.

Note: Repeated freeze–thaw cycles can cause DNA fragmentation, blurred or missing bands, and uneven band intensity, directly compromising the accuracy of electrophoresis results. It is recommended to aliquot and store the sample as needed.

7. How can you quickly troubleshoot blurry bands, smearing, or missing bands after electrophoresis?

Buffer: Replace the electrophoresis buffer with a fresh one; ion imbalance in the old buffer can degrade separation performance.

Gel: Use freshly prepared agarose gels to avoid improper gel concentrations and uneven gelation.

Product: Avoid nuclease contamination and DNA degradation caused by repeated freeze–thaw cycles.

Operation: Control the sample loading volume and verify that the positive and negative terminals are not reversed.

8. How long is the product warranty? How are quality issues handled?

This product is covered by a one-year quality warranty from the date of purchase, and all reagents have undergone rigorous quality testing. In the event of quality issues such as missing bands or degradation, please contact our after-sales service department with your proof of purchase for assistance.

9. What is the core difference between TM1003 and TM1002 (100 bp Min)?

Clip Range: TM1003 covers 100–1500 bp, while TM1002 covers only 100–700 bp.

Highlighted strip: TM1003 bright band at 700 bp, TM1002 bright band at 400 bp;

Applicable scenarios: TM1003 is suitable for identifying PCR products with longer fragments, while TM1002 is designed for rapid analysis of shorter fragments.

10. What are the key precautions for nucleic acid staining and UV observation?

It must be used in conjunction with conventional nucleic acid dyes; the product itself does not contain any staining components.

After staining, visualize the sample using a UV gel imaging system to avoid DNA damage caused by prolonged UV exposure.

Tip: Use a 700-bp bright band as the exposure reference to prevent over- or under-exposure from affecting size determination.