100 bp Mini DNA Ladder (TM1002)
The 100 bp Min DNA Ladder is prepared by blending individually amplified PCR products and consists of 7 distinct DNA fragments. Premixed with loading buffer, it is ready for direct electrophoresis. A recommended loading volume of 6 µl per well is suggested. To facilitate visualization after electrophoresis, the 400 bp band is intensified to appear brightest, with an approximate mass of 100 ng per load, while all other bands are present at approximately 50 ng per load.
| Cat # | Specifications | Unit | Price |
| TM1002-50 | 300 µL | support | ¥160 |
| TM1002-100 | 600 µL | support | ¥320 |
| TM1002-200 | 1200 µL | support | ¥550 |
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Product Highlights
100 bp Min DNA Ladder is a ready‑to‑use molecular weight marker composed of seven individual PCR‑generated DNA fragments.
It is premixed with loading dye for direct gel loading (recommended volume: 6 µl per well).
The 400 bp reference band is present at approximately 100 ng for easy identification, while the remaining bands are each supplied at approximately 50 ng.
The total concentration is approximately 67 ng/µl.
TM1002 | 100 bp Min DNA Ladder
A ready-to-use DNA molecular weight marker premixed with loading buffer, comprising 7 core fragments ranging from 100–700 bp. The 400 bp band is intensified for enhanced visibility, facilitating rapid gel orientation and accurate result interpretation.
Key Features
- Streamlined Band Profile: Contains 7 core fragments for efficient and uncluttered data analysis.
- Enhanced Reference Band: The 400 bp band is doubled in intensity for quick gel anchoring and size estimation.
- Ready-to-Use: Premixed with loading buffer; no additional preparation required prior to loading.
- Quantitative Reference: Fixed DNA mass per band allows for semi-quantitative estimation of sample concentration.
Band Composition
- Number of Bands: 7 distinct DNA fragments
- Fragment Sizes: 100, 200, 300, 400, 500, 600, 700 bp
- Reference Band (400 bp): ~100 ng per loading (Intensified)
- Standard Bands: ~50 ng per loading
Usage Guidelines
- Gel Concentration: 1%–2% Agarose Gel
- Recommended Voltage: 4–10 V/cm
- Suggested Loading Volume: 3–6 µl
- Compatibility: Compatible with standard nucleic acid gel stains/dyes.
TM1002 FAQ
1. How many DNA fragments are included in TM1002? What are the design specifications regarding fragment sizes and band intensity?
This product contains 7 double-stranded DNA fragments, sized at 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, and 700 bp. The 400 bp band is intensified (100 ng per load), while the remaining six bands are each loaded at w50 ng per load, facilitating rapid gel orientation and result interpretation.
2. What is the recommended loading volume? How should I adjust for different well widths?
The standard recommended loading volume is 3–6 µl per well. As a general rule, load 1 µl per 1 mm of well width. For wider wells, increase the loading volume appropriately to ensure sharp, non-diffuse bands.
3. Is loading buffer pre-mixed into this product? Is additional buffer required?
Yes, this product is premixed with loading buffer and requires no additional preparation. After thawing and thorough mixing, it is ready for direct loading and electrophoresis, simplifying your workflow.
4. What are the recommended agarose gel concentrations and electrophoresis voltages?
We recommend using a 1%–2% agarose gel, which is optimal for resolving small DNA fragments ranging from 100–700 bp. Maintain a voltage of 4–10 V/cm (measured as the distance between the electrodes). Avoid excessive voltage, which can cause gel overheating and band distortion.
5. What is the concentration of this product? How is the total DNA mass per load calculated?
The product concentration is approximately 67 ng/µl. At the standard 6 µl loading volume, the total DNA mass is approximately 402 ng (comprising 100 ng for the 400 bp band and 50 ng for each of the other six bands), meeting the requirements for routine visualization and quantitative reference.
6. How should the product be stored? What are the effects of repeated freeze-thaw cycles?
Storage: Store refrigerated or frozen at low temperatures. Do not store at room temperature for extended periods.
Caution: Repeated freeze-thaw cycles can lead to DNA degradation, resulting in fuzzy bands and uneven intensity, which severely impacts electrophoresis results. It is recommended to aliquot the product upon receipt for long-term storage.
7. How do I troubleshoot fuzzy, missing, or smeared bands after electrophoresis?
Buffer: Replace with freshly prepared electrophoresis buffer. Depleted buffer loses ionic strength, causing abnormal separation.
Gel: Use freshly prepared agarose gels to avoid improper concentration or uneven polymerization.
Product: Avoid nuclease contamination and prevent DNA degradation caused by repeated freeze-thaw cycles.
Operation: Control the loading volume to prevent overloading (which causes smearing) and verify that the electrodes are connected correctly.
8. What is the product warranty period? How are quality issues handled?
This product is guaranteed for one year from the date of purchase. All reagents undergo strict quality control testing. In the event of quality issues such as missing bands or degradation, please contact customer support with proof of purchase for assistance.
9. What are the core advantages of TM1002 compared to conventional 100 bp DNA Ladders?
Streamlined Profile: Focuses on 7 core fragments within the 100–700 bp range, eliminating redundant bands for more efficient analysis.
Enhanced Reference: The 400 bp band is specifically intensified for rapid size anchoring.
Ready-to-Use: Premixed with loading buffer, eliminating the need for additional preparation.
Quantitative Accuracy: Fixed DNA mass per band allows for semi-quantitative estimation of target fragments.
10. What precautions should be taken during nucleic acid staining and UV observation?
Staining is required; this product does not contain intercalating dyes. Use with standard nucleic acid gel stains.
Observe under a UV gel imaging system. Avoid prolonged UV exposure to prevent DNA damage.
Tip:Use the intensified 400 bp band as a reference for exposure adjustment to prevent over- or under-exposure, ensuring accurate result interpretation.
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