All

All
Product Management
News
Introduction

Language
- CN
- RU
- EN

Jianshi Biotechnology (Beijing) Co., Ltd.

Small-Volume Total RNA Extraction Kit – 50 (TR205)

● Total RNA (including microRNA) is extracted from any sample that can be lysed using TRIZOL or a similar reagent.
● No phase separation is required, no chloroform is needed, and no precipitation step is necessary.
● The entire process takes only 10 minutes.

Item number	Packaging Specifications	Unit	Price (RMB)
TR205-50	50 times	box	500
TR205-200	200 times	box	1900
TR205-D-50 (contains DNase I)	50 times	box	700
TR205-D-200 (contains DNase I)	200 times	box	2600

Keywords

Scientific instrument

I. Product Overview

The TR205 Small-Volume Total RNA Extraction Kit is a spin-column–based nucleic acid extraction reagent specifically designed for the rapid and efficient isolation of high-quality total RNA, including microRNA. It is compatible with a wide range of biological samples that have been lysed using TRIZOL or similar reagents. This kit eliminates the cumbersome procedures associated with conventional RNA extraction, enabling complete extraction in as little as 10 minutes. The resulting RNA is free from DNA contamination and exhibits excellent integrity, making it directly suitable for a variety of downstream molecular biology applications. In addition, the kit supports automated high-throughput processing, significantly enhancing experimental efficiency.

II. Core Product Features

Characteristic Dimensions	Detailed explanation
Wide range of applications	Supports from Cells, tissues, yeast, plants, bacteria, biological fluids Efficiently extracts total RNA, including microRNA, from all samples that can be lysed using TRIZOL or similar reagents, supporting research applications across multiple fields such as microbiology, botany, and veterinary medicine.
Extremely simple operation流程	RNA extraction can be completed in just three steps—no organic phase separation, no chloroform extraction, and no ethanol precipitation—significantly reducing sample loss and human error, making it easy for even beginners to master.
No genomic DNA contamination	The isolated total RNA is free of genomic DNA contamination and requires no additional DNase treatment, making it directly suitable for downstream applications that are sensitive to DNA contamination, such as qPCR and high-throughput sequencing, thereby eliminating the need for an extra purification step.
Fast, efficient, and time-saving	The entire extraction process takes only 10 minutes , significantly shortening the experimental turnaround time; while also being compatible with automated extraction workflows, it meets the high-throughput processing needs of low-abundance samples and is well-suited for batch-sample experimental settings.

III. Performance Verification Data

1. Validation of High-Integrity RNA Extraction

Using human epithelial cells as the sample, total RNA was extracted with this kit and then subjected to RNA integrity assessment using the Agilent Bioanalyzer (Agilent Technologies Inc.). The results showed that the RNA Integrity Number (RIN) for all samples was greater than 9, demonstrating that this kit provides stable RNA extraction. No significant degradation; excellent integrity High-quality RNA that meets the stringent quality requirements of downstream experimental applications.

2. MicroRNA Recovery and Validation of Quantitative Reliability

This kit enables efficient recovery of microRNAs from samples and supports automated high-throughput extraction. When compared with the miRVana™ (Ambion) small-scale total RNA extraction kit, after recovering total RNA from TRIzol-treated samples, microRNA expression analysis was performed using two mainstream technology platforms:

miRNA-Seq (MiSeq®, Illumina) assay : The number of overlapping miRNAs between the two methods reaches 2,837, with a correlation coefficient for expression. r²=0.9706 , the quantitative results are highly consistent;
nCounter (Nanostring) Direct Hybridization Assay : The number of overlapping miRNAs between the two methods reaches 800, with a correlation coefficient for expression. r²=0.9027 , demonstrating that the microRNA extracted using this kit exhibits high recovery efficiency and highly accurate and reliable quantitative expression results, thereby faithfully reflecting the miRNA expression levels in the sample.

IV. Recommended Downstream Application Scenarios

Real-time fluorescent quantitative PCR (q-PCR/RT-qPCR)
High-throughput sequencing (RNA-seq, small RNA-seq/miRNA-seq)
Gene expression profiling
Quantitative Analysis of MicroRNA Expression and Functional Studies
Transcriptomics research

Core Advantages

Extremely simple operation : No chloroform, phase separation, or isopropanol precipitation required throughout the entire process.
Ultra-fast extraction : Only 10 minutes for the entire journey
High purity : The extracted RNA is free of genomic DNA contamination.
Wide range of applications : Can fully extract small RNAs/microRNAs containing ≥17 nt

Product Specifications

DNase I-free version : TR205-50 (50 times), TR205-200 (200 times)
Includes DNase I version : TR205-D-50 (50 times), TR205-D-200 (200 times)
Maximum binding capacity : CR Purification Column No. 2 can bind up to 50 μg of total RNA
Warranty period : One year from the date of sale

Key Notes

Detergent Preparation : Anhydrous ethanol must be added as required before use.
Avoid contamination : Strictly avoid RNase contamination throughout the entire process.
Sample Preparation : Purification should be performed as soon as possible after lysis to avoid repeated freeze-thaw cycles.
Downstream compatibility : RT-qPCR, NGS, transcriptome analysis, etc.

TR205 Small-Volume Total RNA Extraction Kit – Frequently Asked Questions (10 Items)

1. What types of samples are suitable for the TR205 kit? Is it possible to extract microRNA?

This product is suitable for all samples that can be lysed by TRIzol®-like reagents, including cells, tissues, yeast, plants, bacteria, and biological fluids; it enables the complete extraction of total RNA containing small RNAs/microRNAs of ≥17 nucleotides, thereby meeting the needs for small RNA analysis.

2. Compared with the traditional TRIzol method for RNA extraction, what are the core advantages of TR205?

Extremely simple operation: The entire procedure requires no chloroform, no phase separation, and no isopropanol precipitation, making the operation extremely simple.

Extremely fast: Fast extraction—only 10 minutes from start to finish;

Extremely high purity: The extracted RNA is free of genomic DNA contamination and can be directly used in high-precision assays such as qPCR and NGS.

3. What kit specifications are available? How should I choose between kits with and without DNase I?

Specifications:

No DNase I: TR205-50 (50 reactions), TR205-200 (200 reactions);
Contains DNase I: TR205-D-50 (50 reactions), TR205-D-200 (200 reactions);

Tip: For downstream applications such as qPCR and NGS, which are highly sensitive to DNA contamination, a DNase I–containing formulation is essential; for routine qualitative assays, a DNase-free formulation is sufficient.

4. Must ethanol be added to RNA wash buffer 1/2 before use? What are the specific mixing ratios?

Must be added , Washing solutions without ethanol have no washing effect.

The specific allocation is as follows:

RNA wash solution: 40 mL reagent + 10 mL anhydrous ethanol; or 160 mL reagent + 40 mL anhydrous ethanol.
RNA Wash Solution 2: 12 mL reagent + 48 mL anhydrous ethanol; or 48 mL reagent + 192 mL anhydrous ethanol.

5. What is the standard withdrawal procedure, and how long does it take at the fastest?

Core Process: Lysis and homogenization → addition of an equal volume of anhydrous ethanol → column binding → washing → DNase digestion (optional) → elution;

The entire process takes only 10 minutes. , which is 3–5 times more efficient than conventional RNA extraction methods.

6. What are the reference RNA yield values for different samples? What is the maximum binding capacity of the purification column?

Production reference:

Cells: approximately 10 μg per 10⁶ cells (up to 15 μg for HeLa cells);
Tissue distribution: liver, 40–60 μg/10 mg; muscle, 5–20 μg/10 mg.
Blood: whole human blood, 2–10 μg/mL;

Maximum binding capacity: Maximum binding capacity of CR purification column No. 2 50 μg Total RNA.

7. How can RNase contamination be avoided to ensure RNA integrity and a high RIN value?

Replace gloves throughout the entire procedure and use DNase/RNase-free pipette tips and consumables.

Glassware: bake at 150°C for 4 hours; plasticware: soak in 0.5 M NaOH for 10 minutes, then sterilize.

Prepare solutions using DNase- and RNase-free water, and ensure that the extraction environment is clean and dust-free.

8. How should low RNA purity after extraction (A260/A280 < 1.8) and RNA degradation be investigated?

Low purity (A260/A280 < 1.8): Increase the amount of lysis reagent to ensure complete sample lysis, and strictly follow the washing steps.

Degradation: Prevent RNase contamination, avoid repeated freeze-thaw cycles of the sample, and perform purification as soon as possible after lysis.

Residual ethanol: Spin at idle for 2 minutes to thoroughly remove residual ethanol from the wash solution.

9. How should the components of the kit be stored? What are the effects of repeated freeze-thaw cycles?

Storage conditions:

TRIcom Reagent: Store at 4°C;
DNase I: Store at −20°C;
Other reagents / purification columns: store at room temperature;

Note: Repeated freeze-thaw cycles can reduce DNase I activity, and repeated freezing and thawing of samples can lead to RNA degradation; therefore, it is recommended to aliquot and store DNase I solution after dissolution.

10. What is the product warranty period? Which lysis reagents and downstream assays are compatible?

Warranty: One-year warranty from the date of sale;

Cracking reagent compatibility: Guanidinium-based lysis reagents such as TRIzol®, RNAzol®, QIAzol®, and TriPure™;

Downstream compatibility: All routine RNA assays, including RT-qPCR, NGS sequencing, transcriptome analysis, and molecular hybridization.

TR205

Download