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Jianshi Biotechnology (Beijing) Co., Ltd.

Viral DNA/RNA Extraction Kit TD720

This kit employs an optimized lysis and silica‑membrane purification protocol, eliminating the need for proteinase K or organic solvents such as phenol and chloroform, and ensuring a safe, straightforward workflow. Its unique advantage lies in the integration of EZShield® 2X liquid sample stabilizer, which not only preserves nucleic acids in the sample but also effectively inactivates viruses at room temperature, greatly facilitating sample collection, transport, and storage while enhancing laboratory safety.

Item number	Packaging Specifications	Unit	Price (CNY)
TD720-50	50 times	box	200
TD720-200	200 times	box	700

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GENSTONE BIOTECH’s viral DNA/RNA extraction kit (product catalog number: TD720 series) is a solution specifically designed for the rapid, safe, and high‑quality purification of viral nucleic acids from a wide range of clinical and biological fluid samples.

This kit employs an optimized lysis and silica‑membrane purification protocol, eliminating the need for proteinase K or organic solvents such as phenol and chloroform, and offering a safe and straightforward workflow. Its unique value lies in integrating… EZShield® 2X Liquid Sample Protector This protective agent not only stabilizes nucleic acids in samples but also effectively inactivates viruses at room temperature, greatly facilitating sample collection, transportation, and storage while enhancing the safety of laboratory procedures.

The key features of this kit include:

Broad sample compatibility : Suitable for a wide range of liquid samples, including serum, plasma, cerebrospinal fluid (CSF), whole blood, saliva, oral swabs, stool, urine, and samples treated with the EZShield preservation reagent.

High-purity nucleic acid yield It can simultaneously extract both viral genomic DNA and RNA, yielding nucleic acids of high purity that fully meet the stringent purity requirements of downstream applications such as RT‑PCR, qPCR, next‑generation sequencing (NGS), and nucleic acid hybridization.

Easy and secure operation : The process eliminates the need for phenol–chloroform extraction, thereby obviating the tedious step of organic-phase separation. A proprietary protective agent inactivates potentially infectious samples prior to laboratory entry, reducing biosafety risks.

Efficient purification process : Utilizes a dedicated No. 2 C‑XLR purification column, enabling efficient nucleic acid binding. (Each column can accommodate up to 25 μg of DNA and 50 μg of RNA.) and completes the entire process—from sample lysis to nucleic acid elution—within approximately 15–20 minutes.

Supports ambient-temperature transportation. The standard‑issue EZShield protective agent addresses the critical challenge of requiring viral samples to be transported at low temperatures, providing essential support for remote sampling, epidemiological surveillance, and biobank development.

Main component The kit includes EZShield® 2X liquid sample protection reagent, viral DNA/RNA lysis buffer, viral wash buffer, DNase/RNase‑free water, C‑XLR purification column No. 2, and a 2 mL collection tube.

Downstream applications : The viral DNA/RNA extracted using this kit exhibits high purity and can be directly employed in cutting-edge research and clinical applications, including viral load quantification, genotyping, mutation analysis, molecular diagnostics, and next-generation sequencing (NGS).

Core Advantages

No proteinase K required. : Eliminates the incubation step, making the procedure faster.
Supports EZ Shield® : The protective agent sample can be extracted directly.
High purity : No PCR inhibitors remain, so it can be used directly in downstream experiments.
Store at room temperature All components of the kit should be stored at room temperature.

Support samples

Body fluid sample : Plasma, serum, CSF, whole blood, saliva, urine
Swab sample : Oral swab, nasopharyngeal swab
Other samples : Feces, culture supernatant
Store the sample : Samples preserved with EZ Shield® preservative

Technical Specifications

Extraction time : Total duration 15–20 minutes
Maximum binding capacity : 25 μg DNA / 50 μg RNA
Centrifugal parameters : Room temperature, 10,000–16,000 × g
Sample volume : Maximum ≤400 μl

TD720 Viral DNA/RNA Extraction Kit – Frequently Asked Questions

1. What sample types are supported by this kit? Can samples that have already been preserved with EZ Shield® reagent be directly subjected to extraction?

Supports plasma, serum, CSF, whole blood, saliva, oral swabs, stool, urine, culture supernatants, and samples preserved with EZ Shield® sample preservation reagent;

Samples containing a stabilizing agent require no additional pretreatment and can be directly processed according to the corresponding protocol. The stabilizing agent simultaneously lyses cells, inactivates viruses, and stabilizes nucleic acids, allowing for routine extraction even after ambient‑temperature transport.

2. Is it necessary to use Proteinase K or an organic denaturant during the extraction process? Will omitting them affect the extraction efficiency?

No need to add proteinase K, phenol/chloroform, or other organic denaturants; the kit’s lysis buffer efficiently disrupts viral capsids and fully releases nucleic acids.

By eliminating the proteinase K incubation step and the handling of organic reagents, the assay duration is shortened while avoiding residual organic solvents that could inhibit downstream PCR or sequencing; extraction efficiency and purity remain unaffected.

3. What are the key considerations in preparing viral wash buffer and lysis buffer? What problems can arise from preparation errors?

Lysis buffer: Optional addition of β-mercaptoethanol to a final concentration of 0.5% enhances nucleic acid stability.

Washing liquid: Anhydrous ethanol must be added in proportion (6 ml of wash solution + 24 ml of 100% ethanol; 24 ml of wash solution + 96 ml of 100% ethanol).

Note: Failure to add ethanol can prevent nucleic acids from binding to the column, resulting in extraction failure; excessive addition, on the other hand, reduces the efficiency of washing and leaves residual impurities that inhibit downstream assays.

4. Are there strict requirements for centrifugation parameters and temperature? Will deviations from these parameters affect the results?

All centrifugation steps must be performed at room temperature, at 10,000–16,000 × g.

Excessively low temperatures can reduce elution efficiency, while insufficient centrifugation speeds may cause nucleic acids to fail to bind firmly to the column and result in incomplete washing, thereby directly compromising both yield and purity. It is recommended to strictly adhere to the specified operating parameters.

5. What is the maximum nucleic acid-binding capacity of a single purification column? Can it meet the requirements of high-throughput sequencing and other experiments?

Maximum binding capacity of a single No. 2 C‑XLR purification column 25 μg DNA, 50 μg RNA , with ample yield and high purity of nucleic acids,

It can directly meet the stringent nucleic acid quality requirements of downstream applications such as RT‑PCR, next‑generation sequencing (NGS), and molecular hybridization.

6. What is the purity of the extracted nucleic acids? Are there any residual downstream inhibitors, such as proteins or salt ions?

The kit employs a lysis–column-binding–double-washing protocol combined with ethanol-based residual removal, enabling efficient elimination of proteins, salt ions, and small-molecule inhibitors.

The extracted nucleic acids contain no PCR inhibitors, eliminating the need for additional purification and allowing direct use in highly sensitive molecular assays.

7. How should the kit reagents and the extracted nucleic acids be stored, respectively? What is their shelf life?

Reagent kit storage: All components are stored at room temperature and come with a 1-year warranty from the date of purchase.

Nucleic acid preservation: Eluted viral DNA/RNA: Use immediately; for long-term storage, keep at -70℃ , to prevent nucleic acid degradation caused by repeated freeze-thaw cycles.

8. Compared with other viral extraction kits that require Proteinase K, what are the key advantages of this product?

Time-saving: Omit the proteinase K incubation step at 56°C/70°C; the entire procedure takes approximately 15–20 minutes.

Safety: No organic denaturants, reducing experimental risks and environmental pollution;

Stability: EZ Shield® protective agent enables room-temperature sample transport and inactivates viruses, addressing the challenges of cold-chain logistics.

Convenience: The reagent can be stored at room temperature, eliminating the need for refrigerated storage and transport and reducing usage costs.

9. How can detergent residues be completely removed to prevent inhibition of downstream assays?

After double washing, add an additional 500 μL of 95%–100% ethanol and centrifuge for 1 minute to further remove residual contaminants.

After centrifugation, the tube can be left standing for 1 minute to allow complete removal of residual liquid from the membrane.

Before elution, ensure that the purification column is free of residual liquid to prevent ethanol residues from inhibiting elution and downstream PCR.

10. How should the reaction system be adjusted if the sample volume exceeds 400 μL? What are the core principles guiding this adjustment?

The maximum sample volume compatible with this product is ≤400 μL.

For samples exceeding the recommended volume, proportionally increase the amounts of the protective agent, lysis buffer, and wash buffer while keeping the centrifugation parameters unchanged.

Note: It is strictly prohibited to load an excessive amount of sample onto the column in a single run, to prevent column overload that could result in nucleic acid loss and reduced purity.