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Jianshi Biotechnology (Beijing) Co., Ltd.

Plant/Seed DNA Mini-Prep Kit (TD619, TD620)

The JianShi Bio Plant/Seed DNA Mini‑Extraction Kit is specially designed to rapidly and efficiently isolate high‑quality genomic DNA from a wide range of plant tissues—including leaves, stems, buds, flowers, and fruits—as well as from seeds. Featuring an optimized lysis and purification protocol combined with a proprietary purification column technology, the kit enables the entire workflow—from sample preparation to purified DNA—in approximately 20 minutes. The resulting DNA is free of PCR inhibitors, exhibits high purity, and is suitable for direct use in downstream molecular biology applications such as PCR, restriction digestion, and high‑throughput sequencing.

Item number	Packaging Specifications	Unit	Price (CNY)
TD619-50	50 times	box	200
TD619-200	200 times	box	780
TD620-50 (with an additional inhibitor removal module included)	50 times	box	450

Keywords

Scientific instrument

Related Documents

Product Overview:

The JianShi Bio Plant/Seed DNA Mini‑Extraction Kit is specially designed to rapidly and efficiently isolate high‑quality genomic DNA from a wide range of plant tissues—including leaves, stems, buds, flowers, and fruits—as well as from seeds. It employs an optimized lysis and purification protocol, coupled with a proprietary purification column technology, enabling… Within approximately 20 minutes The entire workflow, from sample preparation to purified DNA, has been completed. The resulting DNA is free of PCR inhibitors, exhibits high purity, and can be directly used in downstream molecular biology applications such as PCR, restriction digestion, and high-throughput sequencing.

Key Product Highlights

Fast and efficient : The entire process takes only about 20 minutes, enabling the extraction of high-quality DNA suitable for PCR from plant samples.

Broad sample compatibility : Suitable for a wide range of plant tissues and seeds, with a maximum processing capacity of up to 150 mg. The product offers two sample‑preparation options—liquid‑nitrogen grinding or bead‑beating in lysis tubes—providing flexibility to accommodate diverse laboratory conditions and sample types.

High purity and high yield : Employing a unique purification strategy, this method effectively removes common inhibitors such as polysaccharides and polyphenols, yielding high‑purity DNA (A260/A280 > 1.8). Typical DNA yields range from 20 to 80 ng per mg of plant material, with up to 25 μg of total DNA recoverable per extraction.

Advanced Pyrolysis System :配套的 Ultra-high-density bead lysis system It exhibits fracture resistance and chemical inertness, enabling efficient, uniform sample disruption and ensuring thorough lysis.

Detailed product features:

Sample Source and Quantity : Leaves, stems, buds, flowers, fruits, seeds, etc.; dosage ≤ 150 mg.

DNA yield Typically 20–80 ng/mg of plant material; the maximum yield per extraction can reach 25 μg.

DNA purity : A260/A280 > 1.8, suitable for sensitive applications such as PCR.

DNA fragment size : Up to or exceeding 40 kb of genomic DNA can be recovered, and in most cases, mitochondrial DNA and viral DNA (if present) can also be recovered.

Operation Time : About 20 minutes.

Downstream applications : PCR, restriction enzyme digestion, cloning, high-throughput sequencing, etc.

Kit Composition and Specifications:

This kit is available in three specifications to accommodate varying throughput requirements:

TD619-50 : 50 reactions

TD619-200 : 200 reactions

TD620-50 : 50 reactions ( Additionally includes an inhibitor removal component. , specifically used to remove inhibitors such as polysaccharides and polyphenols)

The main components include ：

Cracking tube (2.0 mm, supplied as part of the TD620-50 kit)

Lysis buffer

Genomic DNA lysis buffer

Genomic DNA Wash Buffer 1 & 2

Genomic DNA elution buffer

No. 3 F purification column (red, for preliminary filtration)

No. 2 CR purification column (for DNA binding and purification)

Inhibitor Removal Solution & Inhibitor Removal Column ( Only the TD620-50 includes , used for the deep removal of polysaccharides and polyphenols)

2 mL collection tube

(All components are stored at room temperature; specific concentrations vary by specification—see the table in the instruction manual.)

Process Overview

This kit offers two sample preparation protocols, with identical downstream purification steps:

Sample Preparation (Choose One) ：

Scheme A (TD619, liquid nitrogen grinding) : Take a sample of ≤150 mg, thoroughly grind it into a powder using liquid nitrogen, and add lysis buffer to mix well.

Scheme B (TD620, lysate in lysis tubes) : Place the sample, which has been cut into pieces no larger than 150 mg, into a lysis tube, add lysis buffer, and use a homogenizer (e.g., TI2019) to vortex and lyse the sample.

Preliminary purification Centrifuge to remove tissue debris, and transfer the supernatant to… No. 3 F Purification Column , remove large particulate impurities.

DNA Binding and Washing : Add genomic DNA lysis buffer to the filtrate, mix thoroughly, and then pass it through in portions. No. 2 CR purification column , enabling specific binding to DNA. Wash the purification column sequentially with Wash Buffer 1 and Wash Buffer 2 (add ethanol according to the instructions before use).

DNA elution After drying the column membrane, elute with pre-warmed elution buffer to obtain purified DNA.

Deep Depressant (optional, TD620 only) : Pass the eluted DNA through Inhibitor Removal Column It can further remove PCR inhibitors such as polysaccharides and polyphenols, yielding purer DNA suitable for sensitive applications like qPCR and NGS.

Important Notes

Optimization of Sample Preparation For tough samples (such as seeds or rhizomes) or samples rich in polysaccharides and polyphenols (such as strawberries or watermelon), it may be necessary to optimize the homogenization conditions (e.g., by increasing the frequency or duration). The kit recommends using a specific model of homogenizer, such as the TI2019 or TI2023–24.

Reagent Use If low ambient temperatures cause precipitation in the genomic DNA lysis buffer or Wash Buffer 1, heat the solution in a 37°C water bath until it becomes clear before use. β-Mercaptoethanol may be added optionally (final concentration 0.5%) to enhance lysis; DTT (10 mM) can also be used as an alternative.

Recommendations for Special Samples : For plant samples with extremely high levels of polysaccharides and polyphenols (such as strawberries, watermelons, and plant roots and rhizomes), the document recommends using their dedicated Polysaccharide–polyphenol DNA extraction kit (e.g., TB621 magnetic bead method) It may be even more effective.

No RNase A treatment required. This kit selectively binds DNA and, when used within the specified sample volume, does not require RNase A treatment.

Core Advantages

Efficient pyrolysis : Lyophilization or lysis using a grinding instrument to ensure complete sample lysis.
High purity : A₂₆₀/A₂₈₀ > 1.8, with no residual PCR inhibitors
Large DNA fragment : Recyclable ≥40 kb high-molecular-weight genomic DNA
No RNA contamination : Selectively binds double-stranded DNA without the need to add RNase A

Technical Specifications

Sample throughput : Maximum single dose ≤150 mg of plant material
DNA yield : 20–80 ng/mg plant material, with a maximum of 25 μg total DNA
DNA fragment size : ≥40 kb high-molecular-weight genomic DNA
Purity Standard : A₂₆₀/A₂₈₀ > 1.8, suitable for restriction digestion, PCR, and NGS

Key Notes

Pre-processing method : TD619 liquid nitrogen grinding, TD620 grinder for lysis
Inhibitor removal : Only the TD620 is equipped with an inhibitor removal column.
Lysis buffer treatment : Low-temperature precipitation can be dissolved in a 37°C water bath.
Sample Control : Avoid sample overload and ensure complete lysis.

TD619/TD620 Plant DNA Mini‑Extraction Kit – Frequently Asked Questions (10 Key Points)

1. What is the key difference between the TD619 and TD620, and how should you choose between them?

TD619: It employs liquid nitrogen‑based grinding as a pre‑treatment and includes an inhibitor‑free removal kit, making it suitable for routine plant samples.

TD620: Equipped with a lysis tube and a grinder adapter, including an inhibitor‑removal solution and column, it is specifically designed for challenging samples such as those rich in polysaccharides and polyphenols, as well as seeds and rhizomes.

2. What types of plant samples can the kit process? What is the maximum sample volume per run?

Suitable for various plant samples, including leaves, stems, buds, flowers, fruits, and seeds; maximum single‑run processing capacity. ≤150mg , suitable for the sampling needs of most plant species.

3. What are the two methods of sample preparation? How should the grinding parameters be set?

Liquid nitrogen grinding (TD619): After thoroughly grinding the liquid nitrogen–frozen sample into a powder, add lysis buffer and mix well.

Tissue Disruption (TD620):

Vertical oscillation at 60 Hz, 45–60 s per cycle ×2;

Rotate the “8” shape at 6 m/s, with a period of 30 seconds per cycle, repeated twice; for hard seeds, the parameters may be appropriately increased.

4. Is it necessary to add RNase A during the extraction process? Why?

No RNase A needs to be added. The kit employs a selective DNA-binding technology: the purification column binds only double-stranded DNA, while RNA passes through directly, enabling the isolation of genomic DNA free from RNA contamination within the specified sample volume.

5. What is the function of the inhibitor removal column? Is it available only for the TD620?

Only the TD620 is equipped with It is used to remove PCR inhibitors such as polysaccharides, polyphenols, and humic acids from plant samples, thereby enhancing DNA purity and ensuring the smooth execution of sensitive assays like qPCR and NGS; this step may be omitted for routine samples.

6. What should be done if the lysate is too viscous?

1. Reduce the sample volume to avoid overload;

2. Increase the lysis time or enhance the grinding intensity to ensure complete lysis;

3. Add an appropriate amount of genomic DNA lysis buffer to dilute the sample and reduce viscosity.

7. What are the yield, fragment size, and purity of the extracted DNA?

Production: 20–80 ng/mg of plant material; a single elution can yield up to 25 μg of total DNA.

Clip: Recyclable ≥40kb high-molecular-weight genomic DNA;

Purity: A₂₆₀/A₂₈₀ > 1.8, free of PCR inhibitors, suitable for restriction digestion, PCR, and high-throughput sequencing.

8. Can the lysis/washing solution still be used if precipitate forms at low temperatures?

Can be used ; Heat in a 37°C water bath for several minutes until the precipitate is completely dissolved. After cooling to room temperature, it can be used as usual without affecting the extraction efficiency.

9. Is β-mercaptoethanol required? Can it be replaced with other reagents?

Not essential; addition can enhance lysis efficiency. 10 mM DTT may be used as a substitute, and if the sample is thoroughly lysed, this step can be omitted.

10. How can you quickly troubleshoot low DNA yield and PCR amplification failure?

Pre-processing: The sample was not adequately ground or lysed; optimize the grinding time and intensity.

Loading: The mixture was not fully loaded onto the column; multiple injections were performed to ensure complete binding.

Inhibitor: The polysaccharide–polyphenol sample was not subjected to an inhibitor‑removal column; this step has been added.

Ethanol Residue: Insufficient centrifugal force during idle spinning; extend the idle-spinning time to 2 minutes to completely remove ethanol.

TD619, TD620

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Plant/Seed DNA Mini-Prep Kit (TD619, TD620)

TD619/TD620 Plant DNA Mini-Extraction Kit

Core Advantages

Technical Specifications

Key Notes

TD619/TD620 Plant DNA Mini‑Extraction Kit – Frequently Asked Questions (10 Key Points)