All

All
Product Management
News
Introduction

Language
- CN
- RU
- EN

Jianshi Biotechnology (Beijing) Co., Ltd.

Agarose Gel DNA Recovery Kit (TD407)

This product features a unique formulation and a specialized purification column design. At its core, it employs a proprietary ADB solubilization solution to dissolve DNA‑containing gel slices, eliminating the need for organic solvents such as phenol or chloroform and ensuring safe, convenient operation. The resulting lysate is then transferred to a dedicated Type 1 C purification column; through centrifugation, washing, and other steps, DNA purification and recovery can be completed in approximately 15 minutes.

Item number	Packaging Specifications	Unit	Price (CNY)
TD407-50	50 times	box	240
TD407-200	200 times	box	800

Keywords

Core Advantages

Fast and efficient : The entire process takes only 15 minutes to complete the recycling.
Wide-range recycling : Recovers DNA fragments ranging from 50 bp to 23 kb
High recovery rate : Recovery rate for 50 bp–10 kb fragments: 70%–90%
Safety and Environmental Protection : No organic denaturants, chloroform, or other toxic reagents are required.

Product Specifications

50 specification instances : TD407-50 (50 times)
200 specification instances : TD407-200 (200 times)
Maximum binding capacity : Column No. 1, C, can bind up to 5 μg of DNA at maximum.
Sample volume : Maximum single injection volume 800 μl

Key Notes

Detergent Preparation : Anhydrous ethanol must be added as required before use.
Sol conditions : 37–55°C, warm bath for 10 minutes; do not exceed 60°C.
Elution volume : Minimum elution volume ≥10 μl
Downstream compatibility : ligation, sequencing, labeling, PCR, restriction digestion, etc.

TD407 DNA Fragment Purification Kit – Frequently Asked Questions (10 Items)

1. What is the range of recoverable DNA fragments for the TD407? What are the recovery rates for different fragment sizes?

This product is recyclable. 50 bp–23 kb DNA fragment:

50 bp–10 kb fragment : Recovery rate 70%–90%
11 kb–23 kb long fragments : Recovery rate 50%–70%

Meets the needs for routine PCR, restriction enzyme digestion, plasmid fragment recovery, and other applications.

2. How long does the entire recycling process take? What are its key advantages compared to conventional rubber recycling methods?

Operation duration: The entire journey takes only 15 minutes , fast and efficient.

Core Advantages:

No organic denaturants, chloroform, or other toxic reagents are required; the procedure is safe and environmentally friendly.
The unique microcolumn design can elute DNA into ≥10 μl Small size, excellent concentration effect
Purity suitable for high-precision downstream assays

3. Must ethanol be added to the DNA wash solution before use? What are the recommended ratios for different specifications?

Must be added , the wash solution without ethanol shows no impurity removal effect.

Specific ratio:

50 specification instances : 6 ml DNA wash solution + 24 ml anhydrous ethanol (or 26 ml 95% ethanol)
200 specification instances : 24 ml DNA washing solution + 96 ml anhydrous ethanol (or 104 ml 95% ethanol)

4. How can DNA recovery efficiency be maximized, particularly for long fragments greater than 10 kb?

Gel completely dissolved: Incubate at 37–55°C for 10 minutes, with gentle mixing; fragments larger than 8 kb require the addition of an equal volume of water to the gel slice.

Elution Optimization: Preheat the elution buffer to 60–70°C; after sample application, allow it to stand for 2 minutes before centrifugation.

Secondary elution: Long fragments can undergo continuous elution, further increasing the recovery yield.

5. What is the minimum elution volume? What are the key considerations for selecting and handling the eluent?

Minimum elution volume: ≥10 μl

Eluent selection:

Use the DNA elution buffer provided with the kit as a priority.
Elution with water requires maintaining a pH greater than 6.0.

Operating Tips:

Add the eluent directly to the center of the purification column matrix, taking care to avoid contact with the tube walls.
Allow the 6 kb fragment to stand for 1 minute before elution to enhance binding efficiency.

6. What should be done if the DNA purity is low after recovery (A260/A230 ratio is too low) and ethanol residues remain?

Low purity (A260/A230 ratio is low): Avoid contact between the tip of the purification column and the waste liquid in the collection tube to prevent salt contamination.

Ethanol Residue: After washing, spin at high speed for 2 minutes to thoroughly remove residual ethanol and prevent inhibition of downstream ligation and PCR reactions.

7. What is the loading capacity of C purification column No. 1? How should I handle situations where the sample volume exceeds the limit?

Payload: Maximum binding capacity of C purification column No. 1 5 μg DNA

Sample volume: Maximum single injection volume 800 μl

Excessive sample processing: Load the sample in multiple aliquots, centrifuge, and perform washing steps only after all the liquid has been passed through the column.

8. How should the sol temperature and reaction time be controlled? What problems arise if the gel is not fully dissolved?

Optimal sol conditions: 37–55℃ warm bath for 10 minutes, Do not exceed 60℃. Prevent DNA denaturation

Consequences of incomplete gel dissolution:

Generation of minute colloidal particles that clog the purification column.
Reduce DNA binding rate
Directly leads to low recycling volumes.

9. What downstream applications can the recovered DNA be directly used for? Is additional purification required?

Downstream applications: The recovered ultrapure DNA can be used directly for:

DNA ligation, sequencing, labeling
PCR, restriction enzyme digestion

Tip: No additional purification is required, and the purity and integrity meet the stringent demands of high-precision experiments.

10. How should each component of the kit be stored? What are the key troubleshooting points when low recovery or abnormal bands occur?

Storage conditions: Store all components at room temperature, and prevent reagents from volatilizing or oxidizing when left open.

Low Recovery Troubleshooting:

Has the gel completely dissolved?
Does the washing solution contain ethanol?
Is the elution procedure performed in accordance with standards?

Strip anomaly troubleshooting:

Does the pH of the elution water meet the standard?
Does the loading buffer acidify and degrade DNA?