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Jianshi Biotechnology (Beijing) Co., Ltd.

Rapid Coomassie Stain (Destain-Free) (JSR-DY1002)

Cat #	Specifications	Unit	Price (CNY)
JSR-DY1002-50	50ml	bottle
JSR-DY1002-100	100ml	bottle
JSR-DY1002-500	500ml	bottle

Keywords

Jianshi Bio JSR-DY1002 Rapid Coomassie Stain (Destain-Free)

Product Overview

JSR-DY1002 is a modified, rapid Coomassie Brilliant Blue G250 staining solution. It eliminates the need for harsh, toxic reagents commonly found in traditional formulations (such as methanol and acetic acid), utilizing a safer composition consisting solely of Coomassie Brilliant Blue G250, a weak acid, and deionized water. This formula is non-irritating and odorless.
The staining process is performed entirely at room temperature, delivering clear protein band visualization within just 1–2 minutes. It bypasses cumbersome steps such as fixation, boiling, and prolonged decolorization, significantly reducing post-electrophoresis processing time.
This stain is universally compatible with both denaturing and native PAGE gels. The solution is reusable 2–3 times while maintaining stable detection sensitivity. It supports ambient temperature transport and long-term storage at 4°C protected from light. Simple handling protocols are provided for resolving minor precipitation or crystallization issues. Stained gels can be preserved using pure water, glycerol-based solutions, or drying methods.
Intended Use: For research purposes only in protein gel staining. Not for use in clinical diagnostic procedures. Always wear protective gloves and a lab coat during handling.

Product Features

1. Rapid & Efficient Staining
Delivers clear band visualization within just 1–2 minutes at room temperature. Eliminates cumbersome steps such as fixation, boiling, and decolorization, significantly streamlining the protein staining workflow.
2. Safe & Eco-Friendly Formulation
Free from toxic methanol and acetic acid. Composed solely of Coomassie Brilliant Blue G250, a weak acid, and deionized water. Non-irritating and odorless, ensuring a safer laboratory environment.
3. Economical & Reusable
The staining solution can be recycled and reused 2–3 times without compromising detection sensitivity, providing a cost-effective solution for routine protein analysis.
4. Universal Gel Compatibility
Ideal for both denaturing and native PAGE gels. Effectively visualizes low-abundance proteins, ensuring broad applicability across various protein electrophoresis experiments.
5. Convenient Storage & Handling
Supports ambient temperature transport and long-term storage at 4°C protected from light. Simple protocols are provided to resolve minor precipitation or crystallization issues. Stained gels can be preserved using pure water, glycerol-based solutions, or drying methods.

Technical Specifications

Parameter Item	Parameter Details
Product item number and specifications	JSR-DY1002-50 (50 ml), JSR-DY1002-100 (100 ml), JSR-DY1002-500 (500 ml)
Core component	Coomassie Brilliant Blue G250, weak acid, deionized water; no methanol or acetic acid.
Basic dyeing duration	At room temperature, clear protein bands can be observed within 1–2 minutes; the longest staining time may extend overnight.
Applicable gel type	Denaturing protein gel, non-denaturing protein PAGE gel
Number of dye bath reuse cycles	It can be reused 2–3 times after recycling.
Transportation Conditions	Transport at room temperature
Storage conditions	Store at 4°C, protected from light and in a sealed container.
Product expiration date	12 months
Crystallization/Precipitation Treatment	Melt at 37°C in a water bath; if a small amount of precipitate forms, simply shake well before use.
Gel storage method	Three approaches: immersion in pure water, treatment with a 20% glycerol–water solution, and preparation of dry gel.
Minimum Detection Reference	Under standard conditions, proteins at or above 5 ng can be visualized; for low-abundance samples, the staining time may be extended to 1 hour or overnight.
Scope of Use Restrictions	For research use only in protein electrophoresis; not for clinical diagnostic use.

Core Advantages

Ultra-fast dyeing Staining at room temperature for 1–2 minutes, with no fixation or destaining required.
Safe and non-toxic Does not contain methanol or acetic acid–based irritant solvents.
Reusable The dye solution can be recovered and reused 2–3 times.
Broad-spectrum compatibility Universal for denatured and non-denatured protein gels

Product Specifications

Item No. 1 JSR-DY1002-50 (50 ml)
Item No. 2 JSR-DY1002-100 (100 ml)
Item No. 3 JSR-DY1002-500 (500 ml)
Validity period Store at room temperature; refrigerate at 4°C for up to 12 months.

Operation Tips

Protection Requirements Wear gloves and a lab coat when performing the procedure.
Exception Handling Melt the crystals in a 37°C water bath.
Gel preservation Three methods: pure water, glycerin, and dry adhesive.
Scope of Application For research use only: protein electrophoresis experiments

JSR-DY1002 Coomassie Brilliant Blue Rapid Staining Solution – Frequently Asked Questions

1: Does this Coomassie Brilliant Blue staining solution, which does not require destaining, contain methanol and acetic acid? How safe is it?

The formulation contains no methanol or acetic acid—irritating organic solvents—and includes only Coomassie Brilliant Blue G250, a mild acid, and deionized water, with no pungent odor.

Note: The dye solution is mildly acidic and slightly corrosive; always wear disposable gloves and a lab coat throughout the procedure.

2: How long does conventional protein gel staining take, and is a destaining step required?

Under room‑temperature conditions, protein bands can be clearly visualized in just 1–2 minutes, eliminating the need for the tedious steps of fixation, boiling, and destaining.

If deeper staining with higher contrast is desired, the incubation time can be extended to 30 minutes or even overnight.

3: Can the staining solution be recovered and reused after use, and how many times can it be reused at most?

After dyeing is complete, the dye solution can be filtered and recovered into its original reagent bottle; it can be reused up to 2–3 times.

Tip: After multiple reuses, the staining speed may slow down slightly, but it does not reduce the sensitivity of protein detection.

4: If the dye solution develops precipitates or crystallizes at low temperatures, can it still be used normally?

A small amount of sediment in the bottle is normal; shake well to disperse it before use for standard staining.

When crystallization occurs during low-temperature storage, place the entire vial of staining solution in a 37°C water bath to fully dissolve; after cooling, it can be used without affecting the staining results.

5: After staining, the bromophenol blue in the gel turns yellow-green. How can the background color be reduced?

Simply immerse the entire gel in deionized water and let it stand; this will reduce the yellow‑green background caused by bromophenol blue without compromising the protein bands.

6: After staining is complete, no protein bands are visible at all. What could be the cause, and how can this be resolved?

In the vast majority of cases, the protein loading amount is insufficient.

Solution: During electrophoresis, BSA protein was added concurrently as a positive control to identify potential issues in sample loading, electrophoresis, and membrane transfer.

7: After staining, the gel background is relatively high. What methods can be used to reduce background impurities?

1. After electrophoresis, increase the number and duration of distilled water washes to remove impurities from the gel in advance.

2. After staining is complete, immerse the gel in distilled water and allow it to stand at room temperature or at 4°C; overnight incubation at 4°C yields the best destaining results.

8: Which protein gels are compatible? How can the colorimetric sensitivity be enhanced for low-abundance thin-gel samples?

Both denatured and native protein PAGE gels are compatible.

When the protein content is below 5 ng and the gel thickness exceeds 1.0 mm, the staining can be extended to 1 hour or overnight; using a heating device can significantly enhance detection sensitivity.

9: What are the various storage options for protein gels after staining?

Three gel preservation methods:

① Short-term immersion in pure water;

② Immerse in a 20% glycerol aqueous solution to prevent the gel from swelling due to water absorption;

③ Long-term archiving allows for the production of dry adhesive for permanent preservation.

10: What are the product’s transportation and storage conditions, and what is its shelf life?

Transportation conditions: Transport at room temperature; no cold chain required.

Storage conditions: Store at 4°C, protected from light, and in a sealed container.

Product shelf life: 12 months; when stored under strict compliance with recommended conditions, staining performance remains stable.