Low EEO Agarose (TH1001-100)

Cat #	Specifications	Unit	Price
TH1001-100	100g	bottle	160

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Product Overview

GenStone Low Electroendosmosis (EEO) Agarose (Cat# TH1001-100) is a high-purity, high-performance agarose specifically engineered for molecular biology applications. Sourced from Gracilariared algae and refined through advanced purification processes, this product exhibits extremely low electroendosmosis (-Mr < 0.15) and minimal sulfate content (≤0.15%). With a high gel strength (>1200 g/cm²) and moderate gelling/melting temperatures, it serves as the ideal choice for nucleic acid (DNA/RNA) electrophoresis, protein analysis, and subsequent gel extraction and purification. It ensures sharp, crisp band resolution with low background noise.

Key Advantages

Superior Electrophoresis Performance: The extremely low electroendosmosis (-Mr < 0.15) ensures accurate migration of nucleic acids in an electric field, yielding straight and clear bands for easier analysis and imaging.

High Strength & Easy Handling: A gel strength exceeding 1200 g/cm² prevents cracking during handling, allowing for the preparation of a wide range of gel concentrations suitable for resolving nucleic acid fragments of various sizes.

Ultra-High Purity, No Interference: Rigorously tested and certified free of DNase, RNase, proteases, and endonuclease/ligase inhibitors, ensuring the integrity of experimental samples and delivering a clean background.

Low Background, High Signal: Controlled sulfate content (≤0.15%) effectively reduces background fluorescence when used with nucleic acid stains (e.g., EtBr, GelRed, SYBR™ Green), thereby enhancing target band signal intensity.

Natural Source & Consistent Quality: Derived from natural red algae with a stable manufacturing process, guaranteeing high batch-to-batch consistency for reliable and reproducible experimental results.

Core Performance Metrics

Electroosmotic value ： -Mr<0.15 , the stripes are straight and do not curve
Gel strength : 1% concentration >1200 g/cm²
Sulfate content ： ≤0.15% , low background fluorescence
Gelation temperature : 1.5% concentration 35-37℃

Technological Advantages

Enzyme-free contamination : DNase, RNase, and protease were all undetectable.
High purity : Extracted from natural red algae, with a standardized purification process
Batch stability : High inter-batch consistency, with no variability in electrophoresis results.
Widely applicable : Compatible with standard nucleic acid electrophoresis, gel extraction, and fragment separation

Key Features

Strip quality : DNA/RNA bands are straight, with sharp edges and no smearing.
Image quality : Low background fluorescence, high signal-to-noise ratio, and clear weak bands
User-friendly : The adhesive is resistant to breakage, exhibits excellent toughness, and ensures smooth cutting and transfer.
Storage stability : Store sealed; shelf life 36 months

TH1001 Low-Electroosmotic Agarose – High-Frequency FAQ (10 Key Points)

1. What type of agarose does TH1001 belong to? What are its key performance specifications?

TH1001 is low-electroendosmosis (LE) agarose, with the following key specifications: Electroosmotic value (-Mr) < 0.15 、 Gel strength > 1200 g/cm² 、 Sulfate ≤ 0.15% , specifically designed for high-precision nucleic acid electrophoresis.

2. What are the practical benefits of low electroosmotic flow (−Mr < 0.15) in nucleic acid electrophoresis?

Low electroosmotic flow can substantially reduce back‑flow during electrophoresis, resulting in DNA/RNA bands that are straighter, less distorted, with sharp edges and no trailing—thereby significantly improving the accuracy of fragment size determination.

3. What is the source of the raw materials for this agarose, and how is its batch-to-batch stability?

The raw material is a natural extract derived from Indonesian red algae of the genus Gracilaria, processed using a standardized purification protocol. This ensures high batch-to-batch consistency and eliminates electrophoretic variability caused by differences in raw materials.

4. What operational advantages does high gel strength (>1200 g/cm²) provide?

The 1% gel exhibits a strength exceeding 1200 g/cm², resists breakage, and boasts excellent toughness, ensuring smoother sample loading, gel cutting, and transfer. It is well-suited for gel recovery and repeated experimental procedures.

5. Does the product contain interfering substances such as DNase, RNase, or proteases?

After rigorous testing, DNase, RNase, protease, and nuclease inhibitors were all undetected. , it does not degrade nucleic acids or inhibit downstream enzymatic reactions, ensuring maximum experimental safety.

6. What is the impact of sulfate levels ≤0.15% on electrophoresis imaging?

The low sulfate content results in reduced background fluorescence, a higher signal-to-noise ratio, and brighter nucleic acid bands, enabling clear visualization of weak bands and enhancing the detection sensitivity of imaging systems.

7. What are the gelation and melting temperatures, respectively? Are they suitable for standard experiments?

Gelation temperature (1.5%): 35–37℃

Melting temperature (1.5%): 87–89℃

The temperature is moderate, making it suitable for the entire workflow of conventional nucleic acid electrophoresis, gel extraction, and fragment separation.

8. What experimental applications is this agarose suitable for?

Applicable to DNA/RNA electrophoretic separation, gel extraction, fragment analysis, PCR product verification, protein electrophoresis It is a standard molecular biology experiment with extremely broad applicability.

9. How should it be stored properly? What is its shelf life?

Storage conditions: Store in a cool, dry place, tightly sealed; protect from moisture and sunlight.
Shelf life: From the date of manufacture 36 months , long-term storage does not affect the gel’s performance.

10. Compared with conventional agarose, what are the core advantages of TH1001?

• Low electroosmotic flow → Stripes are straighter and clearer

• High gel strength → Easy to make glue, not easy to break

• Ultra-high purity → No enzyme contamination, low background

• Natural ingredients → Stable batch processing, compatible with high-end experiments