50 bp DNA Ladder (TM1001)
| Cat # | Specifications | Unit | Price |
| TM1001-50 | 300 µL | support | ¥177 |
| TM1001-100 | 600 µL | support | ¥354 |
| TM1001-200 | 1200 µL | support | ¥600 |
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Product Description (Technical Data Sheet)
50 bp DNA Ladder is a ready-to-use molecular weight marker composed of ten individual PCR-generated DNA fragments.
It is premixed with loading dye for direct gel loading (recommended volume: 6 µl per well).
The 300 bp reference band is intensified to approximately 100 ng for enhanced visibility, while the remaining nine bands are present at approximately 50 ng each.
The total concentration is approximately 92 ng/µl.
TM1001 50 bp DNA Molecular Weight Standard
50 bp DNA ladder in pre-mixed loading buffer, used for determining the molecular weights of small DNA fragments (50–700 bp) in agarose gel electrophoresis.
Product Specifications
- TM1001-50 : 300 μL
- TM1001-100 : 600 μL
- TM1001-200 : 1200 μL
Strip information
- A total of 10 DNA fragments
- Fragment range: 50 bp to 700 bp
- 300 bp band : Each dose is 100 ng.
- The remaining 9 bands : Each dose is 50 ng per strip.
Key Points of Use
- 1× Loading Buffer, pre-mixed
- Recommended 2%–3% agarose gel
- Voltage: 4–10 V/cm
- Compatible with conventional nucleic acid dyes
TM1001 Frequently Asked Questions
It is a 50-bp DNA ladder in pre-mixed loading buffer, composed of individually prepared PCR products, and is used for determining the molecular weights of small DNA fragments (50–700 bp) in agarose gel electrophoresis. It is suitable for routine molecular experiments involving PCR products, restriction‑digested fragments, and other applications.
| Product Specifications | Volume |
|---|---|
| TM1001-50 | 300 μL |
| TM1001-100 | 600 μL |
| TM1001-200 | 1200 μL |
A total of 10 DNA fragments, with fragment sizes ranging from 50 bp to 700 bp: 50 bp, 100 bp, 150 bp, 200 bp, 250 bp, 300 bp, 350 bp, 400 bp, 500 bp, and 700 bp.
300 bp band : Each dose is 100 ng (produces a brighter signal after electrophoresis, facilitating localization)
The remaining 9 bands : Each dose is 50 ng per strip.
No need. The product is pre-mixed with 1× loading buffer; simply open the cap and load directly for electrophoresis—no need to prepare or add buffer yourself.
Standard dosage : Load 6 μL per sample
General Recommendations : 3–6 μL per well can both yield clear bands.
Calculate the volume as 1 μL per 1 mm of sample well width; for wider wells, you may appropriately increase the volume to 3–6 μL, but avoid overloading, which can cause band smearing.
It is recommended to centrifuge briefly at low speed. , sink to the bottom to prevent wall adhesion
Do not heat; no vigorous vortexing required. , to prevent DNA fragments from being damaged or exhibiting uneven concentrations.
Gel concentration : 2%–3% agarose gel (best for separating small DNA fragments)
Voltage conditions : 4–10 V/cm (distance between positive and negative electrodes)
After staining with a standard nucleic acid dye, simply visualize the sample under a UV gel imaging system or a UV transilluminator; it is compatible with commonly used dyes such as EB, SYBR Green, and GelRed.
The 300-bp band is a brightly labeled reference marker, used at a higher concentration (100 ng) to facilitate rapid localization and size estimation; the remaining bands exhibit uniform brightness and do not interfere with molecular-weight determination, which is consistent with the intended design.
Note: The higher brightness of the 300-bp band is a proprietary design feature that facilitates rapid identification during experiments and does not compromise the accurate determination of molecular weights for other bands.
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