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Tuner(DE3) Super Competent Cells

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Chemically Competent Cells

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Product Features & Benefits

 

1. Uniform & Controlled Expression
The lacY1mutation ensures uniform IPTG uptake​ across the entire bacterial population, resulting in highly consistent and controllable protein expression levels.
 
2. Protease Deficient
Engineered with Lon/OmpT double deletions​ to minimize non-specific degradation of the target protein, thereby enhancing product stability.
 
3. Dual Polymerase Compatibility
Co-expresses both T7 RNA polymerase​ (from DE3) and endogenous E. coli RNA polymerase, ensuring broad compatibility with major expression vectors including pET, pGEX, and pMAL.
 
4. High Transformation Efficiency
Demonstrates reliable performance with a heat-shock efficiency of 1.0 ± 0.5 × 10⁸ cfu/μg DNA.

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Jianshi Biotechnology (Beijing) Co., Ltd.
Company Address: Room 401, Building 11, No. 12 Courtyard, Juyuan Middle Road, Mapo Town, Shunyi District, Beijing  
Phone: 86 18640171737
E-mail: gwang@jianshibio.com
Jianshi Biotechnology (Zhejiang) Co., Ltd.
Address: Building 11, No. 36 Xingshun Road, Zhongyi Ningbo Eco-park, Yuyao City, Zhejiang Province 
WhatsApp: 86 18640171737

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