Pre-stained Protein Marker (10–180 kDa) (TM2001)

Compatible with SDS-PAGE electrophoresis and Western blotting; features color‑coded bands in green and orange‑red for easy visual molecular weight determination; ready to use—direct loading without prior sample preparation.

Product Name	Product item number	Packaging Specifications	Price
Pre-stained Protein Marker (10–180 kDa)	TM2001	box	Inquiry for a quote

Keywords

Jianshi Bio Pre-stained Protein Marker (10–180 kDa) (Catalog No.: TM2001)

Product Overview

This pre-stained protein marker (10–180 kDa) comprises ten highly purified, recombinant proteins ranging from 10 to 180 kDa (10, 15, 25, 35, 40, 55, 70, 90, 130, and 180 kDa), with the 10 kDa band appearing green and the 70 kDa band appearing orange‑red. It is suitable for SDS‑PAGE and Western blotting, with molecular weights accurately calibrated against Thermo’s standard markers. This ready‑to‑use product can be loaded directly without prior preparation. Shelf life is 24 months when stored at −20°C and up to 60 months when stored at −80°C. For research use only (RUO); not intended for clinical diagnostic applications.

Product Features

※ Adaptation to multiple experimental scenarios : Suitable for SDS‑PAGE electrophoresis and Western blotting, meeting the needs of protein molecular weight marker analysis;
※ Color feature stripe localization : The 10 kDa band appears as a green stripe, and the 70 kDa band appears as an orange‑red stripe; the molecular weight positions can be quickly identified with the naked eye.
※ The molecular weight results are accurate. : After calibration with Thermo’s standard markers, the molecular weight references are reliable;
※ Ready-to-use, no pre-processing required. : Pre-mixed with 1× SDS‑PAGE loading buffer and containing a reducing agent; thaw, mix well, and load directly—do not boil, dilute, or add additional reducing agent.
※ Ultra-long shelf life : Stored at ‑20°C, the shelf life is 24 months; stored at ‑80°C, the shelf life can extend up to 60 months. Avoid repeated freeze–thaw cycles; aliquot as needed.
※ Complete range of components with multiple specifications to choose from. : Available in two packaging sizes—2 × 250 μl and 10 × 250 μl—to accommodate varying experimental volume requirements;
Low-temperature transportation ensures quality. : Ice packs/dry ice for low-temperature shipping; please transfer to the specified storage temperature as soon as possible upon receipt.

Technical Specifications for Pre-stained Protein Markers (10–180 kDa) (All Sizes)

Specification Category	Item number	Product Name	Packaging Specifications
Pre-stained Protein Marker 10–180 kDa	TM2001‑1	Pre-stained Protein Marker (10–180 kDa)	2×250 μl
Pre-stained Protein Marker 10–180 kDa	TM2001‑2	Pre-stained Protein Marker (10–180 kDa)	10×250 μl

Product Specifications and Strips

Molecular weight range : 10–180 kDa, with a total of 10 bands
Feature band : 10 kDa green, 70 kDa orange-red
Item number TM2001‑1 : 2 × 250 μL
Item number TM2001‑2 : 10 × 250 μL

Product Highlights

Ready-to-use : Direct injection, no sample pretreatment required
Accurate calibration : Calibrated using Thermo standard markers
Applicable Experiments : SDS-PAGE, Western Blot
For research purposes only : For research use only; not for clinical use.

Storage and Shelf Life

Store at -20℃ Shelf life: 24 months
Store at -80℃ : Shelf life of 60 months
Transportation Conditions : Ice packs/dry ice for low-temperature transport
Operation Tips : Avoid repeated freeze-thaw cycles; we recommend aliquoting as needed.

TM2001 Pre-stained Protein Marker – Frequently Asked Questions (10 Items)

1: What molecular weight bands are included in the TM2001 pre-stained protein marker? Which two bands are the characteristic colored ones?

Molecular weight range: 10–180 kDa; a total of 10 pre-stained recombinant proteins.

The bands are: 10 kDa, 15 kDa, 25 kDa, 35 kDa, 40 kDa, 55 kDa, 70 kDa, 90 kDa, 130 kDa, and 180 kDa.

Color‑coded marker bands: 10 kDa is green, and 70 kDa is orange‑red, enabling rapid visual estimation of molecular weights.

2: What are the two packaging specifications for TM2001, and what are their corresponding part numbers?

TM2001‑1: 2 × 250 μL;

TM2001‑2: 10 × 250 μL.

The product is a ready-to-use, pre-stained protein marker, pre‑formulated with 1× SDS‑PAGE loading buffer and containing a reducing agent.

3: What are the proper storage conditions, shelf life, and transportation requirements?

Shelf life varies with temperature; repeated freeze–thaw cycles are strictly prohibited.

1. Store at ‑20°C: shelf life 24 months; store at ‑80°C: shelf life 60 months.

2. Transportation: Use refrigerated ice packs or dry ice for shipping, and transfer the product to the specified temperature as soon as possible upon receipt.

3. It is recommended to aliquot as needed to minimize repeated freeze–thaw cycles and prevent degradation of banding patterns.

4: Before use, is it necessary to boil, dilute, or add an additional reducing agent?

Important Taboos : Do not boil, do not dilute, and do not add additional DTT or β‑mercaptoethanol.

This product has been formulated in a 1× SDS‑PAGE loading buffer containing a reducing agent and can be loaded directly.

Boiling, dilution, and the addition of extra reducing agents can lead to band smearing and dye loss, rendering the experiment invalid.

For use, simply thaw at room temperature and gently mix thoroughly.

5: What is the standard sample loading amount? How should the sample loading amount be adjusted when using Coomassie Brilliant Blue staining?

Load a standard 5 µL sample (compatible with 5×1.5 mm gel wells) and run electrophoresis concurrently with the samples.

For Coomassie Brilliant Blue staining, the sample loading amount should be reduced; it is recommended to decrease it to 1/5–1/3 of the original volume.

Selection Tips : For first-time use, we recommend conducting a preliminary experiment to determine the optimal sample loading amount for your specific system, thereby conserving consumables and optimizing imaging quality.

6: In low-concentration gel electrophoresis, why do low-molecular-weight bands tend to migrate ahead of the dye front?

This is a common phenomenon with pre-stained markers. Under low-concentration gel conditions, small-molecular-weight proteins such as 10 kDa and 15 kDa migrate rapidly and may migrate ahead of the dye front.

The experiment can appropriately increase the gel concentration and shorten the electrophoresis time to improve the resolution of small-molecule bands.

7: During Western blot transfer, high-molecular-weight bands show poor transfer efficiency—how should this be addressed?

For high-molecular-weight proteins (130 kDa, 180 kDa), it is recommended to extend the transfer time or increase the transfer voltage.

If the results are suboptimal, the transfer buffer formulation can be optimized: reduce the methanol concentration and add a small amount of SDS, ensuring that the final SDS concentration does not exceed 0.1%.

8: Does the apparent molecular weight of a pre-stained marker change? Why does it not exactly match the values of an unstained marker?

Pre-stained proteins bind to the dye, causing shifts in their apparent molecular weights under different buffer and gel systems.

If a precise molecular weight is required, it is recommended to pre‑run a non‑prestained protein marker under the same experimental conditions to establish a calibration curve before estimating the molecular weight.

9: What experiments are supported by the TM2001 pre-stained protein marker? Can it be used for clinical testing?

Applicable experiments: SDS‑PAGE gel electrophoresis and Western blotting, used as a protein molecular weight standard.

This product For research use only , It may not be used for clinical diagnosis or clinical testing.

10: Compared with conventional pre‑stained markers, what are the core advantages of TM2001?

Dual-color feature strip: green + orange-red, for rapid visual localization.
The molecular weights were calibrated using Thermo standard markers, ensuring high accuracy.
Ready-to-use—no boiling or solution preparation required; simply load the sample for immediate use.
Ultra-long shelf life: can be stored at ‑80°C for up to 60 months.

Selection Tips For routine protein electrophoresis and Western blotting, TM2001 pre-stained protein markers are recommended.