M-MLV Reverse Transcriptase II (JSR-E-502)

The enzyme maintains robust activity across a broad temperature range of 42–60°C, exhibits excellent thermal stability and inhibitor tolerance, and efficiently reverse-transcribes RNA with complex secondary structures, while delivering high sensitivity, high specificity, and strong polymerization capability. It is suitable for applications such as first-strand cDNA synthesis, RT‑PCR, and real-time fluorescent quantitative RT‑PCR.

Keywords

Jianshi Bio M-MLV Reverse Transcriptase II (JSR-E-502)

Product Overview

This product is M‑MLV Reverse Transcriptase II (an improved version of M‑MLV reverse transcriptase, catalog number JSR‑E‑502 series), expressed and purified in recombinant E. coli carrying the gene for the modified M‑MLV reverse transcriptase. The enzyme concentration is 200 U/μL, and it is supplied with a dedicated 5× RT reaction buffer. The enzyme maintains robust activity over a broad temperature range of 42–60°C, exhibits excellent thermal stability and tolerance to inhibitors, and efficiently reverse-transcribes RNAs with complex secondary structures, while delivering high sensitivity, high specificity, and strong polymerization capability. It is suitable for first-strand cDNA synthesis, RT‑PCR, real-time fluorescent quantitative RT‑PCR, and other applications. The product is available in three sizes—10 μL, 50 μL, and 200 μL—and should be stored at −25°C to −15°C, protected from light and kept sealed. This product is intended solely for research use only (RUO) and is not for clinical diagnostic purposes.

Product Features

Recombinant, engineered M‑MLV reverse transcriptase, expressed and purified in E. coli, exhibits high purity, stable activity, and excellent batch-to-batch consistency.
Wide temperature activity range: maintains robust reverse transcription activity from 42 to 60°C, which can reduce RNA secondary structures and enhance the efficiency of reverse transcription from complex templates.
The inhibitor tolerance is excellent, enhancing resistance to residual nucleic acid extraction inhibitors in samples and making it suitable for complex reaction systems such as those involving crude‑extracted samples.
It exhibits excellent thermal stability and retains enzymatic activity under high-temperature conditions, thereby enhancing primer‑binding specificity and reducing non‑specific reverse transcription.
High-sensitivity detection enables efficient reverse transcription even with low-abundance RNA templates, making it suitable for cDNA synthesis from trace samples and rare transcripts.
Strong polymerization and extension capability, enabling the synthesis of long‑fragment cDNA products, making it well suited for reverse transcription experiments targeting full‑length transcripts.
Standard unit definition: calibrated at 37°C using PolyA‑Oligo(dT) as the template, with a high concentration of 200 U/μL, ensuring clear and controllable enzyme activity metrics.
The 5× RT Buffer has been optimized for compatibility, with a well‑established reaction‑mix formulation that allows you to prepare the reaction solution directly without any additional optimization.
Three packaging sizes are available: the 10 μL format is ideal for small‑scale preliminary experiments, while the 50 μL and 200 μL formats are suited for routine and high‑throughput assays.
It has a wide range of applications, covering mainstream reverse transcription–related assays such as first-strand cDNA synthesis, RT‑PCR, and real-time fluorescent quantitative RT‑PCR.

M-MLV Reverse Transcriptase II Technical Specification Details (All Specifications)

Specification Category	Item number	Product Name	Packaging Specifications	Storage conditions
M-MLV Reverse Transcriptase II, 10 μL per vial	JSR-E-502-10	M-MLV Reverse Transcriptase II (200 U/μL), with 5× RT Buffer included	Enzyme 10 μL + 5× RT Buffer 100 μL	Store in a dark, sealed container at -25°C to -15°C.
M-MLV Reverse Transcriptase II, 50 μL per vial	JSR-E-502-50	M-MLV Reverse Transcriptase II (200 U/μL), with 5× RT Buffer included	Enzyme 50 μL + 5× RT Buffer 300 μL	Store in a dark, sealed container at -25°C to -15°C.
M-MLV Reverse Transcriptase II, 200 μL pack	JSR-E-502-200	M-MLV Reverse Transcriptase II (200 U/μL), with 5× RT Buffer included	Enzyme 200 μL + 5× RT Buffer 1200 μL	Store in a dark, sealed container at -25°C to -15°C.