10× TBE Electrophoresis Buffer (JSR-EP-1002)

◇TBE has a high buffering capacity, enabling it to maintain stable pH conditions over extended electrophoresis times or at higher voltages. The TBE buffer system is well suited for separating short DNA fragments and DNA fragments of similar sizes, and is widely used in PAGE and high-resolution nucleic acid electrophoresis. It is a commonly employed buffer in DNA polyacrylamide gel electrophoresis and can be used in both native PAGE and certain denaturing PAGE applications. ◇This product is a 10× concentrated stock solution; for experimental use, simply dilute it 1:10 to obtain a 1× TBE working solution.

Keywords

Jianshi Bio 10× TBE Electrophoresis Buffer (JSR-EP-1002)

Product Overview

This product is a 10× TBE electrophoresis buffer (catalog number JSR‑EP‑1002 series), precisely formulated from Tris, boric acid, and EDTA, and serves as a highly concentrated nucleic acid electrophoresis stock buffer. When diluted at a 1:10 ratio, it yields a 1× TBE working solution, suitable for both agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE). The TBE buffering system offers high buffering capacity, ensuring stable pH control during prolonged or high‑voltage electrophoresis, with excellent resolution for separating short DNA fragments, making it ideal for high‑resolution electrophoresis and various PAGE applications. Available in 50 mL and 500 mL sizes, store at room temperature away from light. If slight crystallization occurs during long-term storage, simply mix gently at room temperature to restore usability. Each batch undergoes rigorous quality testing and is intended solely for research use (RUO); it is not for clinical diagnostic purposes.

Product Features

A high-buffering-capacity system ensures stable pH maintenance during prolonged or high-voltage electrophoresis, delivering excellent electrophoretic stability.
The small‑fragment nucleic acid separation offers high resolution, making it well suited for resolving DNA fragments of similar molecular weights, with outstanding high‑resolution electrophoresis performance.
Compatible with dual electrophoresis systems, supporting both agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE).
Compatible with a variety of PAGE assays, it can be used for both native PAGE and certain denaturing PAGE applications in nucleic acid electrophoresis.
10× concentrated stock solution; simply dilute 1:10 to prepare the working solution, saving storage space and ensuring convenient preparation.
Two packaging sizes are available: 50 mL for small‑scale preliminary experiments, and 500 mL for routine high‑throughput laboratory use.
Store at room temperature away from light; no refrigeration or cold-chain requirements, reducing laboratory storage and transportation costs.
Good storage stability; slight crystallization may occur upon long-term storage, but the product can be used normally after being mixed at room temperature.
Accompanied by standardized operating guidelines, it specifies dilution ratios and water‑use requirements, highlights contraindications for borate‑based protective agents and the reuse of buffer solutions, and helps mitigate operational risks and experimental errors.
Rigorous molecular biology‑grade quality control; intended for research use only; stable and reliable performance.

Technical Specification Details for 10× TBE Electrophoresis Buffer (All Specifications)

Specification Category	Item number	Product Name	Packaging Specifications	Storage conditions
10× TBE electrophoresis buffer, 50 mL	JSR-EP-1002-50	10× TBE electrophoresis buffer, high-concentration stock solution, specifically for nucleic acid electrophoresis.	50 mL/bottle	Store at room temperature, protected from light.
10× TBE electrophoresis buffer, 500 mL	JSR-EP-1002-500	10× TBE electrophoresis buffer, high-concentration stock solution, specifically for nucleic acid electrophoresis.	500 mL/bottle	Store at room temperature, protected from light.

Core Performance Features

10× highly concentrated Stock solution; dilute 1:10 to obtain a 1× standard working solution.
High buffer capacity , long-term electrophoresis maintains pH stability and delivers high separation resolution
Standard formula : Tris–boric acid–EDTA system, with stable performance
Applicable Scenarios : Small-fragment nucleic acid isolation, high-resolution PAGE electrophoresis

Item Number, Specifications, and Composition

JSR-EP-1002-50 : 50 mL small size, suitable for small-scale experiments
JSR-EP-1002-500 : 500 mL large format, compatible with high-throughput electrophoresis
Core component : Tris, boric acid, EDTA
System type : Weakly alkaline buffer system with moderate ionic strength

Storage and Safety Regulations

Storage conditions : Store at room temperature, sealed and protected from light.
Slight crystallization : After warming to dissolve and mixing thoroughly, it can be used as usual.
Safety Warning : Contains boric acid; avoid contact with skin and eyes.
Usage Restrictions : For research use only; not for clinical diagnosis.

High-Frequency FAQ on 10× TBE Electrophoresis Buffer

1: What is the concentration factor of this 10× TBE electrophoresis buffer? How do you prepare a standard 1× TBE working solution?

This product is a 10× concentrated stock solution; simply dilute it according to the specified ratio to prepare the standard electrophoresis working solution, making the procedure straightforward.

1. Dilution ratio: Dilute at a volume ratio of 1:10, i.e., 1 part 10× TBE concentrate + 9 parts deionized water.

2. Preparation example: Measure 50 mL of 10× TBE concentrate, add 450 mL of deionized water, and mix thoroughly to obtain a 1× TBE working solution.

3. Usage: After preparation, it can be used directly for agarose gel and polyacrylamide gel casting, as well as for adding to electrophoresis buffer solutions.

Important Reminder This product is a concentrated solution; it must not be used directly in electrophoresis experiments and must be diluted according to the specified ratio.

2: Compared with TAE buffer, what are the key performance differences of TBE buffer? And in which experimental settings is each buffer best suited?

The core differences between the two lie in buffer capacity, migration rate, and downstream compatibility; choose based on your needs.

1. Cushioning performance : TBE has a higher buffering capacity and can better maintain pH stability during prolonged electrophoresis or at high voltages; TAE has lower buffering capacity, making it prone to pH drift during extended electrophoresis.

2. Separation characteristics : TBE offers higher resolution for separating small DNA fragments, making it well suited for distinguishing between fragments of similar molecular weights; TAE electrophoresis provides faster migration and shorter run times.

3. Applicable Scenarios TBE is suitable for separating small DNA fragments and for high-resolution PAGE electrophoresis; TAE is ideal for rapid electrophoresis, DNA gel recovery, and downstream enzymatic reaction assays.

3: What electrophoresis experiments can this product be used for? Why is TBE commonly used in polyacrylamide gel electrophoresis?

TBE is a classic buffer system for polyacrylamide gel electrophoresis and is also compatible with agarose gel electrophoresis, meeting the nucleic acid separation needs of diverse applications.

1. Agarose gel electrophoresis: Suitable for separating small DNA fragments; it offers superior resolution compared to TAE and is ideal for the precise separation of fragments smaller than 2,000 bp.

2. Polyacrylamide gel electrophoresis: It can be used for native PAGE and partially denaturing PAGE experiments and is a commonly employed buffer system for nucleic acid PAGE.

3. Reasons for prioritizing TBE in PAGE: it offers high buffering capacity, maintains a stable pH during prolonged electrophoresis, ensures uniform ionic strength within the gel, delivers high resolution, and enables better separation of nucleic acid fragments of similar sizes.

4: 10× TBE exhibits slight crystalline precipitation upon long-term storage. Is it still usable? What are the storage requirements?

1. Mild crystallization is a normal phenomenon resulting from the decrease in solute solubility at low temperatures: Allow the reagent to stand at room temperature, or gently warm it in a water bath and stir thoroughly until the crystals have completely dissolved and the solution has become clear again; the product can then be used as usual.

2. Standard storage conditions: Store at room temperature, in a sealed container, and protected from light. After use, tightly screw on the cap to prevent evaporation and contamination of the reagent.

3. Disposal Determination: If obvious turbidity, substantial irreversible precipitation, abnormal color, or other irregularities are observed, continued use is not recommended.

Storage Tips : The specific storage temperature and shelf life shall be subject to the information indicated on the product bottle label.

5: What precautions should be taken when using TBE buffer? Can buffers of different concentrations be used interchangeably in equal volumes?

Proper usage is key to ensuring electrophoresis performance and operational safety, with particular attention required for three categories of issues: concentration, safety, and contamination.

1. Concentration standard : Electrophoresis buffers of different concentrations (e.g., 10×, 50×) should not be substituted for one another in equal volumes; they must be diluted according to the appropriate ratio before use. For gel casting and electrophoresis, it is recommended to use TBE from the same batch and at the same concentration.

2. Security Protection : TBE contains boric acid and is irritating; avoid contact with eyes and skin during handling, and if contact occurs, rinse immediately with plenty of water.

3. Usage Guidelines It is not recommended to reuse running buffer after prolonged electrophoresis, as a decline in buffering capacity can compromise separation efficiency. During handling, ensure that containers and pipettes remain free from microbial and nuclease contamination.

4. Use Restrictions This product is intended solely for research and related experimental purposes and shall not be used for human clinical diagnosis or treatment.