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40% Acrylamide/Bis-acrylamide (37.5:1) (JSR-EP-1003)

The acrylamide/bis-acrylamide ratio is fixed at 37.5:1, which helps ensure consistent gel‑making results across different batches. A 40% high‑concentration stock solution allows for the preparation of PAGE gels of varying concentrations through simple calculations, reducing the number of weighing steps required by laboratory personnel and offering broad applicability.

Item number	Packaging Specifications	Unit	Price (CNY)
JSR-EP-1003-50	50 ml	bottle	46
JSR-EP-1003-100	100 ml	bottle	70
JSR-EP-1003-200	200 ml	bottle	108
JSR-EP-1003-500	500 ml	bottle	208

Keywords

Jianshi Bio 40% Acrylamide/Bis-acrylamide (37.5:1) (JSR-EP-1003)

Product Overview

This product is a 40% acrylamide/bis‑acrylamide (37.5:1, catalog number JSR‑EP‑1003 series) high‑concentration monomer stock solution specifically designed for polyacrylamide gel electrophoresis. It is precisely formulated from acrylamide and bis‑acrylamide at a mass ratio of 37.5:1, with a total monomer concentration of 40% (w/v). A fixed crosslinking ratio ensures consistent gel pore sizes across batches, delivering excellent reproducibility in electrophoresis results. The high‑concentration stock solution can be diluted as needed to prepare PAGE gels of varying concentrations, eliminating the need for solid‑form weighing and reducing operational errors and dust contamination. It is compatible with Tris buffer, SDS, APS, TEMED, and other reagents, making it suitable for protein and nucleic acid polyacrylamide gel electrophoresis experiments. Available in 50 mL, 200 mL, and 500 mL volumes, this product should be stored protected from light at 2–8°C and is intended solely for research use (RUO). Acrylamide monomers are neurotoxic and must be handled by trained personnel.

Product Features

With a fixed crosslinking ratio of 37.5:1, the gel pore size exhibits high batch-to-batch consistency, and electrophoretic separation delivers excellent reproducibility.
A 40% high-concentration stock solution that can be diluted as needed to prepare PAGE gels of varying concentrations, eliminating the need for solid‑form weighing.
Formulated with high-purity monomeric raw materials, it exhibits low gel background and no impurity interference, delivering excellent protein/nucleic acid separation performance.
Three packaging sizes are available: 50 mL for small‑scale preliminary experiments, and 500 mL for high‑throughput routine applications.
Precise formulation allows direct calculation of the gel’s total concentration and crosslinking degree, making experimental design both convenient and controllable.
Store at 2–8°C away from light; the reagent exhibits excellent stability, maintaining uniform composition and resisting degradation during long-term storage.
Reduce exposure to solid acrylamide dust, lower health risks for laboratory personnel, and enhance operational safety.
Compatible with conventional Tris‑based electrophoresis systems, it is suitable for a variety of applications, including SDS‑PAGE, native PAGE, and nucleic acid electrophoresis.
Accompanying safety operating guidelines are clearly defined, individual compound toxicity risks are labeled, and laboratory protective measures and operational procedures are standardized.
Rigorous molecular biology–grade quality control; intended for research use only; stable and reliable performance.

Technical Specification Details for 40% Acrylamide/Bisacrylamide (All Specifications)

Specification Category	Item number	Product Name	Packaging Specifications	Storage conditions
40% Acrylamide/Bis-acrylamide 50 mL	JSR-EP-1003-50	40% Acrylamide/Bis-acrylamide (37.5:1), PAGE gel‑casting stock solution	50 mL/bottle	Store at 2–8°C, protected from light.
40% Acrylamide/Bisacrylamide 200 mL	JSR-EP-1003-200	40% Acrylamide/Bis-acrylamide (37.5:1), PAGE gel‑casting stock solution	200 mL/bottle	Store at 2–8°C, protected from light.
40% Acrylamide/Bis-acrylamide 500 mL	JSR-EP-1003-500	40% Acrylamide/Bis-acrylamide (37.5:1), PAGE gel‑casting stock solution	500 mL/bottle	Store at 2–8°C, protected from light.

Core Performance Features

Precise ratio : Acrylamide : Bis-acrylamide = 37.5:1, with high batch-to-batch consistency
High-concentration reserve : 40% (w/v) total monomer concentration; prepare the gel by diluting as needed.
Easy to operate : Eliminates the need for solid weighing and dissolution, reducing human error.
Widely applicable : Compatible with denaturing and native protein electrophoresis, as well as nucleic acid PAGE.

Item Number, Specifications, and Composition

JSR-EP-1003-50 : 50 mL small size, suitable for preliminary experiments
JSR-EP-1003-200 : 200 mL medium size, standard laboratory usage
JSR-EP-1003-500 : 500 mL large format, high-throughput laboratory
Component content : Acrylamide 38.96%, N,N'-methylenebisacrylamide 1.04%

Storage and Safety Regulations

Storage conditions : Store at 2–8°C, sealed and protected from light.
Toxicity Warning : Acrylamide monomer is neurotoxic; handle with care by trained professionals.
Protection Requirements : Gloves, goggles; perform operations inside a fume hood.
Waste liquid treatment : Dispose in accordance with the regulations for hazardous chemicals.

High-Frequency FAQs on 40% Acrylamide Stock Solution

1: What is the compositional ratio of this product? What does 37.5:1 signify?

This product is a precisely pre‑formulated PAGE gel‑casting stock solution, with fixed ratios and total concentrations to ensure reproducible gel preparation.

1. Mass ratio: Acrylamide and N,N'-methylenebisacrylamide are mixed at a mass ratio of 37.5:1.

2. Total concentration: The total monomer concentration is 40% (w/v), comprising approximately 38.96% (w/v) acrylamide and about 1.04% (w/v) N,N′-methylenebisacrylamide crosslinker.

3. Advantages: The factory employs precise fixed ratios, eliminating human weighing errors and enhancing the consistency of gel results across different batches.

2: How do you prepare PAGE running gels of different concentrations using a 40% stock solution? Please provide an example illustrating the calculation method.

Simply calculate the volume of the stock solution using the dilution formula; the procedure is straightforward and eliminates the need to weigh solid reagents.

1. Calculation formula: Required volume of 40% stock solution = (Target gel concentration × Total gel volume) ÷ 40%;

2. Preparation example: To prepare 100 mL of a 10% SDS-PAGE separating gel, the required volume of stock solution is calculated as follows: (10% × 100 mL) ÷ 40% = 25 mL.

3. Subsequent steps: Add the corresponding buffer, SDS, APS, and TEMED; replenish with water to bring the volume to the target level; mix thoroughly, then pour the gel and allow it to polymerize.

Operation Tips : Add APS and TEMED immediately before use, and after addition, quickly mix thoroughly and pour the gel to prevent premature polymerization.

What are the performance characteristics of a crosslinking ratio of 3:37.5:1, and what separation applications is it suitable for?

A crosslinking ratio of 37.5:1 corresponds to a low degree of crosslinking, resulting in relatively larger gel pore sizes that are well suited for the separation of large biomolecules.

1. Pore-size characteristics: With a low degree of crosslinking, the gel network exhibits relatively large pore sizes, resulting in a milder molecular sieving effect.

2. Gel characteristics: The gel exhibits superior flexibility, is less prone to cracking, and offers greater convenience for procedures such as transfer and staining.

3. Applicable scenarios: separation of high-molecular-weight proteins, native PAGE of native proteins, and PAGE of large nucleic acids, among other experiments.

4: What are the storage conditions for this product? What are the consequences of improper storage?

Low-temperature, light‑protected storage is essential for maintaining reagent performance; leaving it at room temperature will accelerate the self‑polymerization of the monomer.

1. Standard storage conditions: Store sealed and protected from light at 2–8°C; avoid repeated freeze-thaw cycles.

2. Effects of improper storage: When stored at room temperature or exposed to prolonged light, acrylamide monomer is prone to self-polymerization, resulting in a decline in effective concentration and reduced gel‑polymerization performance.

3. Disposal criteria: If the solution becomes noticeably cloudy, forms flocculent precipitates, or turns yellow, it must no longer be used.

5: What are the toxicological risks associated with acrylamide monomer? What protective measures should be observed during handling?

Acrylamide monomer is neurotoxic and poses significant health risks; therefore, proper handling and protective measures must be strictly observed.

1. Personnel Requirements: Only trained laboratory personnel are permitted to operate; untrained personnel are prohibited from handling.

2. Personal Protective Equipment: During handling, disposable gloves and safety goggles must be worn, and a lab coat should be donned to prevent direct contact with skin and mucous membranes.

3. Environmental requirements: Avoid inhalation of aerosols; all operations are recommended to be performed inside a fume hood.

4. Waste Liquid Disposal: Experimental waste liquids shall be collected and disposed of in accordance with regulations for hazardous chemicals; unauthorized dumping is strictly prohibited.

Safety Warning : After gel polymerization, acrylamide monomers undergo crosslinking and curing, significantly reducing toxicity; however, proper disposal remains necessary.

6: What types of polyacrylamide gel electrophoresis experiments can this product be used for?

This product is a universal PAGE gel‑casting stock solution, compatible with various buffer systems to support a wide range of electrophoresis applications.

1. SDS-PAGE denaturing electrophoresis: protein molecular weight determination, purity assessment, Western blot sample preparation, and protein quantification analysis, among others;

2. Native-PAGE non-denaturing electrophoresis: analysis of native protein activity, separation of protein complexes, and identification of enzymatic activity, among other applications;

3. Nucleic acid PAGE electrophoresis: separation and detection of small‑molecule nucleic acids, oligonucleotides, SSR markers, and other nucleic acid fragments.

7: What are the advantages of using a pre‑prepared acrylamide stock solution compared to weighing solid reagents yourself?

The core advantages lie in enhancing experimental efficiency, ensuring result consistency, and improving operational safety.

1. Improved efficiency: Eliminates the steps of weighing, dissolving, and filtering solid acrylamide and bis‑acrylamide, significantly reducing pre‑gel preparation time.

2. Stable results: The factory precisely controls the ratio and concentration, eliminating manual weighing errors and ensuring improved batch-to-batch reproducibility of the gel.

3. Safety Protection: Reduces the risk of direct contact between laboratory personnel and toxic solid acrylamide powder, thereby minimizing operational hazards.

8: What determines the pore size of PAGE gels? How do the total gel concentration and cross-linking degree each influence the separation performance?

The gel pore size is determined jointly by the total monomer concentration (%T) and the crosslinking degree (%C); their combination allows for tuning of the separation range.

1. Total monomer concentration: The higher the concentration, the smaller the gel pore size, making it suitable for separating samples with lower molecular weights; the lower the concentration, the larger the pore size, making it suitable for separating large molecules.

2. Crosslinking degree: The higher the proportion of bisacrylamide, the denser the gel network and the smaller the pore size; a ratio of 37.5:1 corresponds to a low crosslinking degree, resulting in larger pores that are well suited for the separation of large molecules.

3. Optimization principle: Adjust the total concentration and crosslinking degree based on the molecular weight of the target sample to achieve optimal separation resolution.

Selection Recommendations For separating small-molecule proteins and nucleic acids, a high crosslinking ratio of 29:1 is recommended; for separating large molecules, a ratio of 37.5:1 is preferred.