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DL5000 DNA Molecular Weight Standard (TM1007)

Product Name	Item number	Packaging Specifications	Price
DL5000 DNA Molecular Weight Standard	TM1007-50	300 µL	Request for quotation
DL5000 DNA Molecular Weight Standard	TM1007-100	300 µL × 2	Request for quotation
DL5000 DNA Molecular Weight Standard	TM1007-200	300 µL × 4	Request for quotation

Keywords

plasmid DNA miniprep extraction kit oem

Jianshi Bio DL5000 DNA Molecular Weight Standard (TM1007)

Product Overview

This product is the DL5000 DNA Molecular Weight Standard (DNA Marker, catalog number TM1007 series), prepared as a mixture of individually synthesized PCR products. It comprises nine DNA ladder fragments: 5,000 bp, 3,000 bp, 2,000 bp, 1,500 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, and 100 bp, covering a broad range from 100 to 5,000 bp and suitable for analyzing large‑fragment nucleic acids. The product is pre‑mixed with 1× Loading Buffer, making it a ready‑to‑use reagent; simply load 3–6 μL onto the gel for electrophoresis, yielding clear, well‑defined bands. The stock solution has a concentration of approximately 91 ng/μL; at a standard loading volume of 6 μL, the 1,500‑bp and 500‑bp bands each contain about 100 ng, while the remaining fragments each contain roughly 50 ng. Two brightly labeled reference bands facilitate accurate molecular‑weight estimation. The product is available in three packaging sizes. For short‑term storage, keep refrigerated at 4°C away from light (avoid repeated freeze–thaw cycles); for long‑term storage, store sealed at −20°C. Each batch undergoes rigorous nucleic‑acid quality testing, and a one‑year quality warranty is provided from the date of sale. It is suitable for molecular‑weight calibration in agarose gel electrophoresis of double‑stranded DNA, including PCR products, plasmid restriction digests, and long‑fragment amplification products, and is intended solely for research use (RUO).

Product Features

Nine‑segment, full‑coverage gradient strips can accommodate up to 5,000 bp of large fragments, balancing short‑fragment amplification with the analysis of plasmid restriction‑enzyme digestion products, thus broadening their range of applications.
Dual‑highlighted reference bands ensure doubled loading capacity for 1500‑bp and 500‑bp markers, providing clear references across both high‑ and low‑molecular‑weight ranges for more efficient molecular‑weight determination.
Pre-mix with 1× loading buffer; simply load the sample directly out of the box, eliminating the need to prepare and dilute the buffer and streamlining the pre-electrophoresis workflow.
The stock solution maintains a stable concentration of 91 ng/μL, with uniform and consistent band intensity across batches, and exhibits excellent electrophoresis reproducibility.
Flexibly compatible with various sample wells: For standard loading, use 3–6 μL; adhere to the guideline of adding 1 μL per 1 mm well width, and slightly increase the load volume for wider wells.
A universal nucleic acid electrophoresis system, compatible with 1–2% agarose gels; a recommended electrophoresis voltage of 4–10 V/cm; and suitable for standard laboratory electrophoresis equipment.
Standardized low-temperature storage protocol: short-term storage at 4°C and long-term storage at −20°C; seal the vial immediately after use to prevent reagent degradation and loss of potency.
Comprehensive, batch‑by‑batch nucleic acid testing ensures product stability, and an official one‑year full‑scope quality warranty is provided.
Complementary electrophoresis optimization tips: regularly replace the electrophoresis buffer and use fresh gels to minimize artifacts such as smearing, spurious bands, and band blurring.
Comes with a standard electrophoresis band reference map, allowing direct comparison with sample electrophoresis images for rapid and accurate determination of DNA fragment molecular weights.

DL5000 DNA Marker Technical Specifications (All Sizes)

Specification Category	Item number	Product Name	Packaging Specifications
DL5000 DNA Marker, 300 μL per vial	TM1007-50	DL5000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL/vial
DL5000 DNA Marker, 300 μL, pack of two	TM1007-100	DL5000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 2 vials
DL5000 DNA Marker, 300 μL, pack of four	TM1007-200	DL5000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 4 vials

Core Nucleic Acid Parameters

Fragment band : 9 standard bands at 5000/3000/2000/1500/1000/750/500/250/100 bp
Stock solution concentration : Approximately 91 ng/μL; pre-mix with 1× Loading Buffer for immediate use.
Sample loading capacity : 6 μL loaded; 1500 bp/500 bp approximately 100 ng, with the remaining bands at 50 ng.
Applicable Experiment : Long-fragment PCR verification, plasmid restriction digestion, and vector construction by electrophoresis

Full range of item numbers and specifications

TM1007-50 : Single 300 µL basic mini-packaging
TM1007-100 : 2 × 300 µL Medium-Sized Laboratory Kit
TM1007-200 : 4 × 300 µL high-throughput bulk packaging
Warranty Standards : 12-month full warranty from the date of purchase

Electrophoresis and Storage Guidelines

Recommended gel : 1%–2% agarose gel system
Electrophoresis voltage : 4–10 V/cm gel length
Short-term storage : Store temporarily at 4°C away from light; do not store for extended periods.
Long-term storage : Store at -20°C, protected from light; strictly prohibit repeated freeze-thaw cycles.

DL5000 DNA Marker TM1007 Series – Frequently Asked Questions

1: How many DNA fragments are included in the DL5000 DNA Marker, and what are their respective molecular weights? When 6 μL is loaded, what are the nucleic acid contents of each band?

This ultra‑high‑resolution marker panel comprises nine DNA fragments, with a maximum coverage of 5,000 bp. It features dual bands at 1,500 bp and 500 bp, offering enhanced loading capacity and improved resolution for long‑fragment electrophoresis.

1. Full-length fragments: 5,000 bp, 3,000 bp, 2,000 bp, 1,500 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp;

2. Nucleic acid loading: The two bands at 1500 bp and 500 bp each correspond to approximately 100 ng, while the remaining seven molecular-weight bands all correspond to 50 ng.

3. The accompanying electrophoresis schematic clearly resolves 5,000- and 3,000-bp super-large DNA fragments, making it suitable for determining the molecular weights of plasmids and long‑fragment amplification products.

2: What are the part numbers in the TM1007 series, and what are the individual‑unit and total‑volume specifications for each part number?

The entire product line offers three standardized packaging options, tailored to meet the monthly consumption needs of different laboratories:

1. TM1007-50: Individually packaged, with a single‑tube volume of 300 µL;

2. TM1007-100: The kit contains two units, each with a volume of 300 µL, for a total volume of 600 µL.

3. TM1007-200: The kit contains 4 tips, each with a volume of 300 µL, for a total volume of 1200 µL.

Selection Tips : For small research groups, 50 models are available; the plasmid construction and high-throughput cloning lab prioritizes bulk packaging of 200 units to reduce consumable costs.

3: Does the TM1007 DL5000 come with electrophoresis sample loading buffer, or do I need to add a loading buffer separately when using it?

The product is a ready-to-use, pre‑formulated marker. The stock solution is pre‑mixed with 1× Loading Buffer, eliminating the need to prepare buffer yourself; simply open the cap and load directly.

1. Directly pipette 3–6 μL into the gel well to complete the sample loading.

2. The buffer contains an electrophoresis indicator, enabling intuitive monitoring of nucleic acid migration distance and preventing excessive loss of very large DNA fragments during gel electrophoresis.

3. Eliminates the buffer preparation step, reducing the risk of exogenous nucleic acid contamination in long‑fragment samples.

4: What is the nucleic acid concentration of the DL5000 stock solution? What is the recommended loading volume range for routine electrophoresis?

1. The overall nucleic acid concentration of the stock solution is approximately 91 ng/μL, with a homogeneous system free of precipitates and localized concentration or stratification.

2. Recommended sample loading volume range: 3 μL to 6 μL;

3. For low‑copy, long‑fragment PCR products, 6 μL can be used to enhance band intensity; for high‑concentration plasmid restriction‑enzyme digest products, 3 μL is sufficient for clear visualization.

5: What are the correct storage conditions for TM1007 DL5000, and can the reagent be subjected to repeated freeze-thaw cycles?

Ultra‑high molecular weight DNA is highly sensitive to temperature fluctuations; it is advisable to employ separate short‑term and long‑term storage protocols, as repeated freeze–thaw cycles can directly degrade long‑fragment nucleic acids.

1. Short-term temporary storage (to be used within 1 month): Store in a sealed container, protected from light, at 4°C.

2. Long-term storage (more than 1 month): Store at −20°C, protected from light.

Important Taboos Repeated freeze–thaw cycles can cleave long DNA fragments, resulting in the loss of large bands at 5,000 and 3,000 bp and a general blurring and smearing of the bands; the reagents must be discarded and are no longer usable.

6: How long is the warranty period for the TM1007 series DNA markers, and what is the warranty start date and the scope of exclusions?

1. Official warranty period: A full one-year (12-month) quality guarantee is provided from the date of purchase.

2. Warranty Coverage: Under compliant low-temperature storage conditions, requests for after-sales service may be submitted in cases of band loss, nucleic acid degradation, or insufficient concentration.

3. Not covered under warranty: Reagent degradation and failure resulting from storage at elevated temperatures, repeated freeze–thaw cycles, or prolonged exposure to air after opening the container.

7: When using the DL5000 electrophoresis system, what are the recommended agarose gel concentration and electrophoresis voltage settings?

1. Gel concentration compatibility: 1%–2% agarose gels; a 1% low‑concentration gel is suitable for separating ultra‑large fragments of 5,000/3,000/2,000 bp, while a 2% gel is ideal for the precise resolution of small fragments ranging from 100 to 1,000 bp.

2. Electrophoresis voltage control standard: 4–10 V/cm between the gel’s two electrodes;

3. In low-concentration gels, large DNA fragments migrate at a slower pace with well‑defined, smooth bands; in high-concentration gels, small fragments achieve higher resolution, but such gels are not suitable for separating fragments longer than 5,000 bp.

Operation Tips : When the electric field exceeds 10 V/cm, gel heating occurs, leading to smearing and broadening of high‑molecular‑weight bands, making accurate molecular‑weight determination impossible.

8: The sample wells in agarose gels vary in width. How should the loading volume be adjusted for the TM1007 Marker, and is there a standardized reference?

1. Basic conversion standard: For each 1 mm of well width, load 1 μL of DL5000 Marker.

2. For standard electrophoresis gels, a well width of 3–4 mm is recommended, and a typical loading volume of 3–4 μL is sufficient to meet imaging requirements.

3. For preparative-scale gels with large pore sizes, the sample volume may be increased proportionally to the pore width; however, the total volume loaded in a single run should not exceed 10 μL to prevent sample overflow and cross-contamination of adjacent lanes.

9: What operational precautions should be observed when routinely using the TM1007 Marker, as failure to do so may directly compromise the quality of electrophoresis band imaging?

1. Prolonged exposure of the sample to air after opening the cap: nucleic acids volatilize and oxidize, the buffer pH shifts, and the intensity of all bands decreases markedly.

2. Repeated freeze–thaw cycles of reagents: long‑fragment DNA strands degrade, and the prominent 5,000‑bp and 3,000‑bp bands disappear entirely.

3. Reusing expired electrophoresis buffer or long‑stored, aged gels: bands become compressed and overlapped, with severe smearing;

4. Long-term storage at room temperature: Nucleic acids degrade rapidly, rendering the entire reagent vial completely ineffective.

Operating Procedures : Immediately tighten the bottle cap after each reagent withdrawal to minimize exposure to air.

10: How should the DL5000 Marker bands be visualized after electrophoresis? What factors can cause band smearing or the loss of large DNA fragments?

After thorough staining with a nucleic acid dye, the gel can clearly resolve nine bands corresponding to different molecular weights when placed under a UV imaging system, enabling straightforward and accurate determination of very large DNA fragments.

1. Standard observation procedure: electrophoresis completed → staining with nucleic acid dye → imaging and documentation using a UV transilluminator or gel imaging system;

2. Common causes of band smearing and large‑fragment loss: ① Repeated reuse of electrophoresis buffer leading to loss of efficacy; ② Gel concentration too high, resulting in poor resolution of very large fragments; ③ Insufficient sample volume; ④ Repeated freeze–thaw cycles of reagents causing degradation of long DNA fragments; ⑤ Excessively high electrophoresis voltage, leading to gel heating and diffusion.