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DL2000 Plus DNA Molecular Weight Standard (TM1006)

Product Name	Item number	Packaging Specifications	Price
DL2000 Plus DNA Molecular Weight Standard	TM1006-50	300 µL	Request for quotation
DL2000 Plus DNA Molecular Weight Standard	TM1006-100	300 µL × 2	Request for quotation
DL2000 Plus DNA Molecular Weight Standard	TM1006-200	300 µL × 4	Request for quotation

Keywords

Jianshi Bio DL2000 Plus DNA Molecular Weight Standard (TM1006)

Product Overview

This product is the DL2000 Plus DNA Molecular Weight Standard (DNA Marker, catalog number TM1006 series), prepared as a mixture of individually synthesized PCR products. It comprises seven DNA ladder bands: 2000 bp, 1500 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp, with an added 1500‑bp band, providing finer resolution across the 100–2000 bp range. The product is pre‑mixed with 1× Loading Buffer, making it a ready‑to‑use reagent; simply load 3–6 μL for electrophoresis, yielding clear, well‑defined bands. The stock solution has a concentration of approximately 66 ng/μL; at a standard loading volume of 6 μL, the 750‑bp band corresponds to about 100 ng, while each of the other bands contains roughly 50 ng, with the intermediate bands exhibiting enhanced brightness for easy interpretation. The product is available in three packaging sizes. For short‑term storage, keep refrigerated at 4°C away from light (avoid repeated freeze–thaw cycles); for long‑term storage, store sealed at −20°C. Each batch undergoes rigorous nucleic acid quality testing, and a one‑year quality warranty is provided from the date of sale. It is suitable for molecular weight calibration in agarose gel electrophoresis of double‑stranded DNA, including PCR products and plasmid restriction digests, and is intended solely for research use (RUO).

Product Features

Seven gradient‑based, finely tuned fragment sizes are provided, with an additional 1500‑bp band that fills the gap in the standard DL2000 range, delivering enhanced resolution for nucleic acid fragments between 100 and 2000 bp.
Pre-mix 1× sample buffer—ready-to-use and pre‑formulated, eliminating the need to prepare the buffer and streamlining pre‑electrophoresis procedures.
Differentiated band‑loading design doubles the yield of the 750‑bp band and enhances its brightness, enabling rapid alignment with the central reference ladder for more intuitive molecular‑weight determination.
The stock solution maintains a stable concentration of 66 ng/μL, with uniform band intensity across batches and excellent electrophoresis reproducibility.
Flexibly compatible with various gel wells: For standard 3–6 μL sample loading, follow the guideline of adding 1 μL per 1 mm of well width; for wider wells, the sample volume may be increased accordingly.
A universal nucleic acid electrophoresis system, compatible with 1–2% agarose gels, recommends an electrophoresis voltage of 4–10 V/cm, and is suitable for standard laboratory electrophoresis equipment.
Standardized low-temperature storage protocol: store at 4°C for short-term use and at −20°C for long-term preservation; immediately reseal the vial after use to prevent degradation and loss of potency.
Comprehensive nucleic acid-specific quality inspections are conducted on every batch prior to shipment, and a one-year warranty is provided from the date of sale; experimental data are stable and reliable.
Supporting electrophoresis optimization tips: regularly replace the electrophoresis buffer and use freshly prepared gels to avoid abnormal results such as smearing, spurious bands, and band blurring.
Comes with a standard electrophoresis band reference map, allowing direct comparison with sample electrophoresis images for rapid and accurate determination of DNA fragment molecular weights.

DL2000 Plus DNA Marker Technical Specifications (All Formats)

Specification Category	Item number	Product Name	Packaging Specifications
DL2000 Plus DNA Marker, 300 μL per vial	TM1006-50	DL2000 Plus DNA Molecular Weight Standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL/vial
DL2000 Plus DNA Marker, 300 μL, pack of two	TM1006-100	DL2000 Plus DNA Molecular Weight Standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 2 vials
DL2000 Plus DNA Marker, 300 μL, pack of four	TM1006-200	DL2000 Plus DNA Molecular Weight Standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 4 vials

Core Nucleic Acid Parameters

Fragment band : 2000/1500/1000/750/500/250/100 bp, 7 standard bands
Original solution concentration : Approximately 66 ng/μL; pre-mix with 1× Loading Buffer for immediate use.
Sample loading capacity : 6 μL loaded; the 750-bp band corresponds to approximately 100 ng, while the other bands correspond to 50 ng.
Applicable Experiment : Precise identification of PCR products, distinguishing fragments between 1,000 and 2,000 bp.

Full range of item numbers and specifications

TM1006-50 : Single 300 µL basic mini-packaging
TM1006-100 : 2 × 300 µL medium-sized laboratory kit
TM1006-200 : 4 × 300 µL high-throughput bulk packaging
Warranty Standards : 12-month full warranty from the date of purchase

Electrophoresis and Storage Guidelines

Recommended gel : 1%–2% agarose gel system
Electrophoresis voltage : 4–10 V/cm gel length
Short-term storage : Store temporarily at 4°C away from light; do not store for extended periods.
Long-term storage : Store at -20°C, protected from light; strictly prohibit repeated freeze-thaw cycles.

DL2000 Plus DNA Marker TM1006 Series – Frequently Asked Questions

1: How many DNA fragments are included in the DL2000 Plus DNA Marker, and what are their respective molecular weights? When 6 μL is loaded, what are the nucleic acid contents of each band?

This upgraded Marker contains seven DNA fragments and features a newly added 1,500-bp band. Its ability to resolve large DNA fragments outperforms that of the standard DL2000, and at a 6-μL loading volume, the bands exhibit uniform brightness in the gel image.

1. Full fragments: 2000 bp, 1500 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp;

2. Nucleic acid loading: the 750 bp band is approximately 100 ng, while the remaining six bands of different molecular weights are each 50 ng.

3. The accompanying electrophoresis schematic allows for the clear differentiation of adjacent large DNA fragments, addressing the challenge of resolving products in the 1,000–2,000 bp range.

2: What are the part numbers in the TM1006 series, and what are the individual‑unit and total‑volume specifications for each part number?

The entire product line offers three standardized packaging options, tailored to meet the monthly usage requirements of different laboratories:

1. TM1006-50: Individually packaged single unit, with a volume of 300 µL per unit;

2. TM1006-100: The kit contains 2 units, each with a volume of 300 µL, for a total kit volume of 600 µL.

3. TM1006-200: The kit contains 4 tips, each with a volume of 300 µL, for a total kit volume of 1200 µL.

Selection Tips : For small research groups, 50 models are selected; the molecular high-throughput laboratory receives priority for bulk orders of 200 units to reduce procurement costs.

3: Does the TM1006 DL2000 Plus come with electrophoresis sample loading buffer, or do I need to add an additional loading buffer when using it?

The product is a ready-to-use, pre‑formulated marker; the stock solution is pre‑mixed with 1× Loading Buffer, eliminating the need to prepare buffer solutions yourself—simply open the cap and use.

1. Directly pipette 3–6 μL into the gel well to load the sample;

2. The buffer contains an electrophoresis indicator, allowing for intuitive monitoring of nucleic acid migration distance and preventing over‑running.

3. Eliminates the buffer preparation step, reducing the risk of exogenous nucleic acid contamination.

4: What is the nucleic acid concentration of the DL2000 Plus stock solution? What is the recommended loading volume range for routine electrophoresis?

1. The overall nucleic acid concentration of the stock solution is approximately 66 ng/μL, with a homogeneous formulation free of precipitates and localized concentrations.

2. Recommended sample loading volume range: 3 μL to 6 μL;

3. For low‑copy,微量 PCR products, 6 μL can be used to enhance band visibility; for high‑concentration amplification products, 3 μL is sufficient for clear imaging.

5: What are the proper storage conditions for TM1006 DL2000 Plus, and can the reagents be subjected to repeated freeze-thaw cycles?

Nucleic acid molecular weight standards are sensitive to temperature fluctuations; two distinct storage protocols—short-term and long-term—are recommended, as repeated freeze–thaw cycles will directly render the reagents ineffective.

1. Short-term temporary storage (to be used within 1 month): Store in a sealed container, protected from light, at 4°C.

2. Long-term storage (more than 1 month): Store at −20°C, protected from light.

Important Taboos Repeated freeze–thaw cycles can cleave DNA double strands, leading to the loss of small DNA fragments and smearing or trailing of the overall band pattern; the reagent must be discarded and is no longer usable.

6: What is the warranty period for the TM1006 series DNA markers, and what are the warranty start date and the scope of exclusions?

1. Official warranty period: A full one-year (12-month) quality guarantee is provided from the date of purchase.

2. Warranty Coverage: Under compliant low-temperature storage conditions, claims for after-sales service may be submitted in cases of band loss, nucleic acid degradation, or insufficient concentration.

3. Not covered under warranty: Damage to reagents caused by storage at elevated temperatures, repeated freeze–thaw cycles, or prolonged exposure to air after opening the container.

7: When using the DL2000 Plus electrophoresis system, what are the recommended agarose gel concentration and electrophoresis voltage settings?

1. Gel concentration compatibility: 1%–2% agarose gels; a 1% gel is suitable for separating large DNA fragments of 2000/1500/1000 bp, while a 2% gel is ideal for the precise resolution of small fragments ranging from 100 to 750 bp.

2. Electrophoresis voltage control standard: 4–10 V/cm between the gel’s two electrodes;

3. In low-concentration gels, large DNA fragments migrate slowly and produce well‑defined bands; in high-concentration gels, small fragments achieve higher resolution.

Operation Tips : When the electric field exceeds 10 V/cm, gel heating occurs, leading to band broadening and smearing.

8: The sample wells in the agarose gel are of inconsistent width. How should the loading volume of the TM1006 Marker be adjusted, and is there a standardized reference?

1. Basic conversion standard: For each 1 mm of well width, load 1 μL of DL2000 Plus Marker.

2. The standard electrophoresis gel well width is 3–4 mm, and a typical sample volume of 3–4 μL is sufficient.

3. For preparative-scale columns with large pore sizes, the sample volume may be increased proportionally to the pore width; however, the total volume per injection should not exceed 10 μL to prevent sample overflow and cross-contamination of adjacent lanes.

9: What operational precautions should be observed when routinely using the TM1006 Marker, as failure to do so may directly compromise the quality of electrophoresis band imaging?

1. Prolonged exposure of the sample to air after opening the cap: nucleic acids volatilize and oxidize, the buffer pH shifts, and the intensity of all bands decreases markedly.

2. Repeated freeze–thaw cycles of reagents: DNA strands degrade, and low‑molecular‑weight bands at 100/250 bp disappear entirely.

3. Reusing expired electrophoresis buffer or long‑stored, aged gels: bands become compressed and overlapped, with severe smearing;

4. Long-term storage at room temperature: Nucleic acids degrade rapidly, rendering the entire reagent vial completely ineffective.

Operating Procedures : Immediately tighten the bottle cap after each aliquot to minimize exposure to air.

10: How do you visualize the DL2000 Plus Marker bands after electrophoresis? What factors can cause band smearing or poor visibility?

After thorough staining with a nucleic acid dye, the gel can clearly resolve seven bands corresponding to different molecular weights when placed under a UV imaging system, enabling intuitive and accurate determination of molecular weights.

1. Standard observation procedure: electrophoresis is completed → nucleic acid dye–mediated staining → imaging and documentation using a UV transilluminator or gel imaging system;

2. Common causes of band smearing: ① Repeated reuse of electrophoresis buffer leading to loss of efficacy; ② Gel concentration mismatched with the target fragment; ③ Insufficient sample volume; ④ Repeated freeze–thaw cycles causing nucleic acid degradation; ⑤ Excessively high electrophoresis voltage resulting in gel overheating.