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DL2000 DNA Molecular Weight Standard (TM1005)

Product Name	Item number	Packaging Specifications	Price
DL2000 DNA Molecular Weight Marker	TM1005-50	300 µL	Inquiry for a quote
DL2000 DNA Molecular Weight Marker	TM1005-100	300 µL × 2	Inquiry for a quote
DL2000 DNA Molecular Weight Marker	TM1005-200	300 µL × 4	Inquiry for a quote

Keywords

Jianshi Bio DL2000 DNA Molecular Weight Standard (TM1005)

Product Overview

This product is the DL2000 DNA Molecular Weight Standard (DNA Marker, catalog number TM1005 series), prepared as a mixture of individually synthesized PCR products. It comprises six DNA ladder bands at 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp, covering the typical molecular‑biology nucleic acid analysis range of 100–2000 bp. The product is pre‑mixed with 1× Loading Buffer, making it a ready‑to‑use reagent; simply load 3–6 μL for electrophoresis, yielding clear, well‑defined bands. The stock solution has a concentration of approximately 58 ng/μL; at a standard loading volume of 6 μL, the 750‑bp band corresponds to about 100 ng, while each of the other bands contains roughly 50 ng, with the intermediate bands exhibiting distinct brightness for easy interpretation. The product is available in three packaging sizes. For short‑term storage, keep refrigerated at 4°C away from light (avoid repeated freeze–thaw cycles); for long‑term storage, store sealed at −20°C. Each batch undergoes rigorous nucleic acid quality testing, and a one‑year quality warranty is provided from the date of sale. It is suitable for molecular‑weight calibration in agarose gel electrophoresis of double‑stranded DNA, including PCR products and plasmid restriction digests, and is intended solely for research use (RUO).

Product Features

Six gradient standard fragments provide full coverage, making them suitable for electrophoretic analysis of nucleic acid fragments in the vast majority of 100–2000 bp size ranges.
Premix 1× loading buffer; ready-to-use formulation eliminates the need to prepare the buffer, simplifying experimental procedures.
Differentiated band‑intensity design ensures higher brightness for the 750‑bp band, enabling rapid alignment with the central reference ladder and facilitating more convenient molecular‑weight determination.
The reagent concentration is stable at 58 ng/μL, with excellent batch-to-batch consistency and outstanding electrophoretic band reproducibility.
Flexible and adjustable sample-loading protocol: Typically load 3–6 μL, following the guideline of adding 1 μL per 1 mm gel well width, to accommodate wells of various sizes.
A universal electrophoresis-compatible system, compatible with 1–2% agarose gels; a recommended electrophoresis voltage of 4–10 V/cm; and compatible with standard nucleic acid electrophoresis equipment.
Standardized storage guidelines: short-term storage at 4°C and long-term storage at −20°C, with corresponding sealed‑container protection requirements to prevent reagent volatilization and oxidation‑induced degradation.
We have enhanced after-sales quality assurance, conducting nucleic acid testing on every batch before shipment and offering a one-year warranty, ensuring high reliability of test results.
Supporting electrophoresis optimization tips: it is recommended to replace the electrophoresis buffer regularly and use fresh gels to avoid abnormal electrophoresis results such as smearing and spurious bands.
Comes with a standard electrophoresis band reference map, allowing direct comparison with sample electrophoresis results for rapid determination of DNA fragment sizes.

DL2000 DNA Marker Technical Specifications (All Sizes)

Specification Category	Item number	Product Name	Packaging Specifications
DL2000 DNA Marker, 300 μL per vial	TM1005-50	DL2000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL/vial
DL2000 DNA Marker, 300 μL, pack of two	TM1005-100	DL2000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 2 vials
DL2000 DNA Marker, 300 μL, pack of four	TM1005-200	DL2000 DNA molecular weight standard, pre-mixed with 1× Loading Buffer, ready to use.	300 μL × 4 vials

Core Nucleic Acid Parameters

Fragment band : 2000/1000/750/500/250/100 bp, 6 standard bands
Stock solution concentration : Approximately 58 ng/μL, pre-mixed with 1× Loading Buffer
Recommended sample loading : Typically 3–6 μL; at 6 μL, approximately 100 ng of a 750-bp fragment.
Applicable Experiment : Identification of standard PCR products by agarose gel electrophoresis

Full range of item numbers and specifications

TM1005-50 : Single 300 µL basic pack
TM1005-100 : 2 × 300 µL in a medium batch format
TM1005-200 : 4 × 300 µL, laboratory bulk packaging
Warranty Standards : Full 12-month quality warranty from the date of sale

Electrophoresis and Storage Guidelines

Recommended gel : 1%–2% agarose gel system
Electrophoresis voltage : 4–10 V/cm gel length
Short-term storage Store at 4°C away from light; do not store for extended periods.
Long-term storage : Freeze at -20℃; do not subject to repeated freeze-thaw cycles.

DL2000 DNA Marker TM1005 Series – Frequently Asked Questions

1: What DNA fragments are included in the DL2000 DNA Marker? And when 6 μL is loaded, what are the nucleic acid contents of each band?

This Marker contains six sets of standard DNA fragments; with a 6 μL loading volume, the band intensity remains consistent, and the electrophoresis bands are uniformly clear.

1. Includes fragments of: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp;

2. Nucleic acid load: the 750-bp band is approximately 100 ng, while the other five bands are each 50 ng.

3. The accompanying electrophoresis reference chart allows for the intuitive differentiation of bands corresponding to various molecular weights, facilitating the determination of PCR product sizes.

2: What are the part numbers in the TM1005 series, and what are the corresponding package volume specifications for each part number?

The entire series offers three standardized packaging options, tailored to meet the varying usage requirements of different laboratories:

1. TM1005-50: Individually packaged single unit, with a volume of 300 µL per unit;

2. TM1005-100: The kit contains two units, each with a volume of 300 µL, for a total volume of 600 µL;

3. TM1005-200: The kit contains 4 tips, each with a volume of 300 µL, for a total volume of 1200 µL;

Selection Tips : Small research groups may select 50 models, while high-throughput molecular laboratories are given priority for bulk orders of 200 models.

3: Does the TM1005 DL2000 DNA Marker come with a loading buffer, or do I need to add a separate loading buffer when using it?

The product is a ready-to-use, pre‑formulated marker; the stock solution already contains a 1× loading buffer, so no additional buffer preparation is required.

1. After opening the cap, simply pipette 3–6 μL directly into the gel well to simplify the experimental procedure.

2. The buffer contains a built-in indicator, allowing for intuitive monitoring of nucleic acid migration during electrophoresis.

3. No separate preparation of glycerol or dye‑buffer solutions is required, reducing the risk of reagent contamination.

4: What is the nucleic acid concentration of the DL2000 DNA Marker stock solution? What is the recommended loading volume range for routine experiments?

1. The overall nucleic acid concentration of the stock solution is approximately 58 ng/μL, with uniform distribution and no localized concentration or precipitation.

2. Recommended sample volume: 3 μL to 6 μL;

3. Low-concentration,微量 PCR products can be loaded at 6 μL, while high-concentration products require only 3 μL to produce clear bands.

5: What are the proper storage conditions for the TM1005 DL2000 DNA Marker, and can it be subjected to repeated freeze-thaw cycles?

Nucleic acid–based reagents are sensitive to temperature fluctuations; strict separation of short-term and long-term storage protocols is required, and repeated freeze–thaw cycles are prohibited.

1. Short-term temporary storage (to be used within 1 month): Store in a refrigerator at 4°C, protected from light.

2. Long-term storage (more than 1 month): Store at –20°C, protected from light.

Important Taboos Repeated freeze–thaw cycles can cause DNA strand breaks, resulting in the loss of small DNA fragments and blurred, smeared bands; such samples must be discarded.

6: How long is the warranty period for the TM1005 series DNA markers, and how is the warranty start date determined?

1. Official warranty period: A full one-year (12-month) quality guarantee is provided from the date of purchase.

2. Warranty Coverage: Under compliant storage conditions, strip loss, nucleic acid degradation, and insufficient concentration are all eligible for after-sales service.

3. Human error (such as repeated freeze-thaw cycles, storage at high temperatures, or prolonged exposure with the container open) is not covered under the warranty.

7: When using the DL2000 Marker for electrophoresis, what are the recommended agarose gel concentration and electrophoresis voltage settings?

1. Gel matrix: 1%–2% agarose gel; within this range, the six bands exhibit optimal resolution.

2. Electrophoresis voltage control: 4–10 V/cm between the gel’s two electrodes;

3. Low-concentration gels (1%) are suitable for separating large DNA fragments, while 2% gels are ideal for the precise resolution of small fragments in the 100–750 bp range.

Operation Tips : Excessively high voltage can cause the gel to overheat and drag the bands, while excessively low voltage results in an overly slow separation rate.

8: When the wells of an agarose gel vary in width, how should the sample loading volume be adjusted, and are there any established guidelines to follow?

1. Basic conversion standard: For each 1 mm of well width, load 1 μL of marker.

2. For standard 96-well plates, the well width is 3–4 mm, and a typical volume of 3–4 μL is sufficient.

3. For preparative-scale columns with large pore sizes, the sample volume may be increased proportionally, up to a maximum of 10 μL, to prevent sample overflow.

9: What operational precautions should be observed when using the TM1005 Marker in routine applications, as improper use can directly compromise the quality of electrophoresis bands?

1. Prolonged exposure of the cap to air: nucleic acids may volatilize and oxidize, pH may shift, and the overall band intensity may diminish.

2. Repeated freeze–thaw cycles: DNA degrades, and low-molecular-weight bands disappear entirely.

3. Reusing expired electrophoresis buffer or long‑stored, aged gels: bands exhibit smearing and overlapping.

4. Long-term storage at room temperature: Nucleic acids rapidly degrade and become inactivated, rendering the entire vial unusable.

Operating Procedures : Tighten the cap immediately after each use to minimize exposure to air.

10: How do you visualize the DL2000 Marker bands after electrophoresis? What factors can cause the bands to appear blurred or indistinct?

After staining with a nucleic acid dye, the gel clearly resolves six bands of varying molecular weights under UV illumination, and the procedure is simple and intuitive.

1. Standard observation procedure: After electrophoresis is completed, immerse the gel in nucleic acid staining solution for staining, then place it under a UV transilluminator or imaging system to capture images.

2. Common causes of band smearing: ① Repeated cycling of the electrophoresis buffer leading to loss of efficacy; ② Gel concentration mismatched with the DNA fragment size; ③ Insufficient sample volume; ④ Repeated freeze–thaw cycles causing nucleic acid degradation; ⑤ Excessively high electrophoresis voltage.