BrightFISH® Nucleic Acid Stain (JSR-DY1004)

Item number	Specifications	Unit	Price (CNY)
JSR-DY1004-100	100ul	pipe	98
JSR-DY1004-500	500ul	pipe	278

Keywords

Jianshi Bio BrightFISH ^® Nucleic acid dye JSR-DY1004

Product Overview

BrightFISH® nucleic acid dye is a 10,000× stock solution fluorescent staining reagent, offering a safe alternative to the highly toxic EB for nucleic acid visualization. The reagent has been rigorously validated by both the Ames test and zebrafish assays as non‑mutagenic and incapable of penetrating intact cell membranes. It is excited by 488 nm blue light, eliminating the need for UV exposure—protecting laboratory personnel while minimizing damage to nucleic acid samples. The product withstands boiling at 100°C and exhibits excellent long‑term stability at room temperature; it can detect as little as 3 ng of DNA without interfering with normal electrophoretic migration. Even at high loading levels, bands remain clear and well‑defined, and it is compatible with both agarose gels and PAGE polyacrylamide gels. Post‑electrophoresis, no destaining step is required, and the staining working solution can be reused up to three times when stored away from light. Gels prepared using the gel‑staining method can be stored at room temperature in the dark for 5–7 days. When used with JianShi Bio’s proprietary DNA markers, it avoids band distortion and smearing, making it ideal for a wide range of nucleic acid electrophoresis applications in research laboratories. This product is intended solely for research use and is not approved for clinical diagnostics or applications in the food and pharmaceutical sectors.

Product Features

Safe, low-toxicity, and non‑mutagenic: validated for safety using both the Ames test and zebrafish assays; unable to penetrate intact cell membranes; blue‑light imaging eliminates the need for UV illumination, thereby avoiding UV‑induced damage; serves as a non‑toxic alternative to ethidium bromide.
Exceptional stability: supports long-term storage at room temperature, withstands boiling at 100°C, and exhibits no significant performance degradation upon repeated use.
Ultra-high detection sensitivity: capable of detecting as little as 3 ng of DNA, with clear banding even for low-concentration nucleic acids.
Excellent electrophoresis band resolution: does not interfere with DNA migration rates; even at high loading amounts, bands remain sharp without smearing or distortion; compatible with both agarose and PAGE gels.
Simple and time-saving: No destaining is required after electrophoresis, and the staining solution can be stored away from light and reused up to three times.
Wide compatibility with imaging equipment: Excited by a 488 nm laser, it is perfectly matched to blue-light gel‑cutter systems and blue-light gel scanners, and is also compatible with UV imaging systems.
Flexible and convenient to use: The precast gel with dye can be stored at room temperature, protected from light, for 5–7 days, and when used with JianShi’s proprietary DNA marker, it produces clean, well-defined bands.

Technical Specifications

Parameter Item	Parameter Details
Product item number and specifications	JSR-DY1004-100 (100 μL), JSR-DY1004-500 (500 μL)
Dye concentration	10,000× aqueous solution
Excitation wavelength	488 nm
Minimum DNA detection limit	3 ng
Applicable gel type	Agarose gel, polyacrylamide PAGE gel
High-temperature resistance	Can withstand boiling at 100°C.
Number of reuse cycles for the dye bath	Can be reused up to 3 times under light-protected conditions.
Shelf life of gel-based dyes during storage	Store at room temperature, protected from light, for 5–7 days (it is recommended to prepare and use immediately).
Recommended Observation Instruments	Blue-light gel cutter and blue-light scanner; compatible with UV imaging (image brightness is relatively low).
Storage and Transportation Conditions	Transport at room temperature; store protected from light at room temperature.
Scope of Application	Nucleic acid electrophoresis experiments for research purposes are prohibited for clinical diagnosis and the use in food and pharmaceutical products.

Core Advantages

Safe and low-toxicity Dual experimental validation shows no mutagenicity, and blue-light imaging causes no UV‑induced damage.
High sensitivity Minimum detectable 3 ng trace DNA sample
High-temperature stability Can be boiled at 100°C and stored at room temperature for long periods.
Colorfast No destaining is required after electrophoresis, and the staining solution can be reused.

Product Specifications

Dye concentration 10,000× aqueous solution
Item No. 1 JSR-DY1004-100 (100 μL)
Item No. 2 JSR-DY1004-500 (500 μL)
Excitation wavelength 488nm

Precautions for Use

For research purposes only Not for clinical use or use in the food and pharmaceutical sectors.
Operational Protection Wear gloves and a lab coat at all times.
Storage method Store at room temperature, protected from light.
Recommended dyeing The glue-dyeing and dip-dyeing methods are not recommended; spot dyeing is also not advised.

Super GelBlue Nucleic Acid Stain – Frequently Asked Questions

1: How safe is Super GelBlue nucleic acid stain, and can it replace EB?

It can completely replace the highly toxic EB, offering significant safety advantages:

1. Confirmed to be non-mutagenic through dual validation in the Ames assay and zebrafish.

2. Dyes cannot penetrate intact cell membranes, thereby reducing the risk of human absorption;

3. Imaging is excited by 488 nm blue light, eliminating the need for UV irradiation and protecting both operators and nucleic acid samples.

2: Does Super GelBlue dye support spot‑dyeing?

The dot‑staining method is not recommended. It requires precise calculation of the ratios among the dye, the loading buffer, and the nucleic acid, making the procedure cumbersome and yielding inconsistent staining results; gel‑based staining or soaking‑in staining are preferred.

3: How long can agarose gels prepared by the gel‑dye method be stored, and what are the recommended usage guidelines?

It can be stored for 5–7 days at room temperature, protected from light. To ensure clear electrophoresis bands and experimental reproducibility, it is recommended to prepare and use the solution fresh.

4: When running electrophoresis with DNA markers from other brands, how can band distortion and smearing be addressed?

This dye is incompatible with all commercially available Marker brands and is highly prone to banding artifacts.

Solution: If this issue occurs, please contact JianShi Bio’s technical support for a compatible solution. We recommend using JianShi Bio’s proprietary DNA marker to avoid band distortion and smearing.

5: Can this dye be visualized using a UV gel imaging system? Which type of imaging equipment is recommended?

Compatible with ultraviolet imaging systems, but in UV mode the strip brightness is low, resulting in poor imaging quality.

It is ideally compatible with blue-light gel imaging systems and blue-light scanners (excited at 488 nm), delivering clear images while minimizing the risk of UV‑induced damage.

6: Can the dye solution used for soaking and dyeing be reused? What are the storage requirements?

The dyeing working solution should be stored away from light and can be reused approximately three times.

Note: Reused staining solutions must be stored away from light, as exposure to light can cause the dye to degrade prematurely, reducing staining sensitivity.

7: Is Super GelBlue heat‑stable? Will boiling agarose during gel preparation degrade the dye?

The product exhibits exceptional stability, withstanding boiling at 100°C; when preparing gels, adding dyes during microwave‑assisted high‑temperature melting of agarose does not compromise their performance. It also supports long‑term storage at room temperature, maintaining consistent performance without degradation.

8: What is the lowest amount of DNA that can be detected with this dye? Which gels are compatible?

Limit of detection: 3 ng of trace DNA sample;

Applicable gel: Agarose gels and polyacrylamide PAGE gels are both suitable for routine nucleic acid electrophoresis.

9: What are the transportation and storage conditions for Super GelBlue?

Store at room temperature, protected from light. The expiration date is indicated on the outer packaging label.

10: The gel stained with dye exhibits uneven coloration upon visual inspection. Does this affect electrophoresis experiments?

It is normal for the gel to exhibit varying shades of color between the top and bottom; this does not affect DNA electrophoretic mobility or band visualization and has no impact on the final experimental results, so electrophoresis can proceed as usual.